Dissociated G?GTP and G?? Subunits are the Major Activated Form of Heterotrimeric Gi/o Proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F14%3A00420933" target="_blank" >RIV/67179843:_____/14:00420933 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Dissociated G?GTP and G?? Subunits are the Major Activated Form of Heterotrimeric Gi/o Proteins

Popis výsledku v původním jazyce

While most heterotrimeric G proteins are thought to dissociate into G? and G?? subunits upon activation, the evidence in the Gi/o family has long been inconsistent and contradictory. The Gi/o protein family mediates inhibition of cAMP production and regulates activity of ion channels. Based on experimental evidence, both heterotrimer dissociation and rearrangement have been postulated as crucial steps of Gi/o protein activation and signal transduction. We have now investigated the process of Gi/o activation in living cells, directly by two-photon polarization microscopy and indirectly by observations of G protein-coupled receptor kinase-derived polypeptides. Our observations of existing fluorescently labeled and non-modified G?i/o constructs indicate that the molecular mechanism of G?i/o activation is affected by the presence and localization of the fluorescent label. All investigated non-labeled, non-modified Gi/o complexes extensively dissociate upon activation. The dissociated subun

Název v anglickém jazyce

Dissociated G?GTP and G?? Subunits are the Major Activated Form of Heterotrimeric Gi/o Proteins

Popis výsledku anglicky

While most heterotrimeric G proteins are thought to dissociate into G? and G?? subunits upon activation, the evidence in the Gi/o family has long been inconsistent and contradictory. The Gi/o protein family mediates inhibition of cAMP production and regulates activity of ion channels. Based on experimental evidence, both heterotrimer dissociation and rearrangement have been postulated as crucial steps of Gi/o protein activation and signal transduction. We have now investigated the process of Gi/o activation in living cells, directly by two-photon polarization microscopy and indirectly by observations of G protein-coupled receptor kinase-derived polypeptides. Our observations of existing fluorescently labeled and non-modified G?i/o constructs indicate that the molecular mechanism of G?i/o activation is affected by the presence and localization of the fluorescent label. All investigated non-labeled, non-modified Gi/o complexes extensively dissociate upon activation. The dissociated subun

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

—

Svazek periodika

289

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

1271-1281

Kód UT WoS článku

000332401700007

EID výsledku v databázi Scopus

—