Dissociated G alpha(GTP) and G beta gamma Protein Subunits Are the Major Activated Form of Heterotrimeric Gi/o Proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F14%3A43887351" target="_blank" >RIV/60076658:12310/14:43887351 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.jbc.org/content/289/3/1271.full" target="_blank" >http://www.jbc.org/content/289/3/1271.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1074/jbc.M113.493643" target="_blank" >10.1074/jbc.M113.493643</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dissociated G alpha(GTP) and G beta gamma Protein Subunits Are the Major Activated Form of Heterotrimeric Gi/o Proteins

Popis výsledku v původním jazyce

The nature of the activated form of heterotrimeric Gi/o proteins is unclear.Non-modified Gi/o heterotrimers dissociate upon activation, whereas fluorescently labeled Gi/o heterotrimers dissociate or rearrange depending on fluorescent protein localization.Dissociated G(GTP) and G subunits represent the major activated form of Gi/o proteins.The identification of the Gi/o protein activated form is crucial for the elucidation of signaling mechanisms and rational drug design. Although most heterotrimeric G proteins are thought to dissociate into G and G subunits upon activation, the evidence in the Gi/o family has long been inconsistent and contradictory.The Gi/o protein family mediates inhibition of cAMP production and regulates the activity of ion channels. On the basis of experimental evidence, both heterotrimer dissociation and rearrangement have been postulated as crucial steps of Gi/o protein activation and signal transduction. We have now investigated the process of Gi/o activation i

Název v anglickém jazyce

Dissociated G alpha(GTP) and G beta gamma Protein Subunits Are the Major Activated Form of Heterotrimeric Gi/o Proteins

Popis výsledku anglicky

The nature of the activated form of heterotrimeric Gi/o proteins is unclear.Non-modified Gi/o heterotrimers dissociate upon activation, whereas fluorescently labeled Gi/o heterotrimers dissociate or rearrange depending on fluorescent protein localization.Dissociated G(GTP) and G subunits represent the major activated form of Gi/o proteins.The identification of the Gi/o protein activated form is crucial for the elucidation of signaling mechanisms and rational drug design. Although most heterotrimeric G proteins are thought to dissociate into G and G subunits upon activation, the evidence in the Gi/o family has long been inconsistent and contradictory.The Gi/o protein family mediates inhibition of cAMP production and regulates the activity of ion channels. On the basis of experimental evidence, both heterotrimer dissociation and rearrangement have been postulated as crucial steps of Gi/o protein activation and signal transduction. We have now investigated the process of Gi/o activation i

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Svazek periodika

289

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1271-1281

Kód UT WoS článku

000332401700007

EID výsledku v databázi Scopus

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