Quantitation of mRNAs for M(1) to M(5) subtypes of muscarinic receptors in rat heart and brain cortex.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F02%3A20020035" target="_blank" >RIV/67985823:_____/02:20020035 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Quantitation of mRNAs for M(1) to M(5) subtypes of muscarinic receptors in rat heart and brain cortex.
Popis výsledku v původním jazyce
It has been generally accepted that, of the five subtypes of muscarinic receptors (M(1)-M(5)), only the M(2) subtype is expressed in mammalian heart. This notion has recently been challenged by a series of reports indicating that mRNAs for some or all non-M(2) subtypes are also present in mammalian heart, in parallel with the M(2) mRNA. However, the quantities of relevant mRNAs reported to be present in the heart are not known, which makes it difficult to evaluate their likely significance. We measuredthe concentrations of the five muscarinic mRNAs by competitive reverse transcription-polymerase chain reaction and discovered that the M(2) mRNA represents more than 90% of total muscarinic mRNAs in rat atria and in either ventricle. The concentrations of total muscarinic mRNAs and of the M(2) mRNA were more than twice as high in the atria than in the ventricles. mRNAs for all non-M(2) muscarinic receptor subtypes were also detected but represented less than 1% (M(1) and M(4)), less than
Název v anglickém jazyce
Quantitation of mRNAs for M(1) to M(5) subtypes of muscarinic receptors in rat heart and brain cortex.
Popis výsledku anglicky
It has been generally accepted that, of the five subtypes of muscarinic receptors (M(1)-M(5)), only the M(2) subtype is expressed in mammalian heart. This notion has recently been challenged by a series of reports indicating that mRNAs for some or all non-M(2) subtypes are also present in mammalian heart, in parallel with the M(2) mRNA. However, the quantities of relevant mRNAs reported to be present in the heart are not known, which makes it difficult to evaluate their likely significance. We measuredthe concentrations of the five muscarinic mRNAs by competitive reverse transcription-polymerase chain reaction and discovered that the M(2) mRNA represents more than 90% of total muscarinic mRNAs in rat atria and in either ventricle. The concentrations of total muscarinic mRNAs and of the M(2) mRNA were more than twice as high in the atria than in the ventricles. mRNAs for all non-M(2) muscarinic receptor subtypes were also detected but represented less than 1% (M(1) and M(4)), less than
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FH - Neurologie, neurochirurgie, neurovědy
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/IAA7011910" target="_blank" >IAA7011910: Receptory pro neuromediátory a přenos signálů v srdci. Jejich modulace vlivem glukokortikoidů.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2002
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecular Pharmacology
ISSN
0026-895X
e-ISSN
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Svazek periodika
61
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
6
Strana od-do
1267-1272
Kód UT WoS článku
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EID výsledku v databázi Scopus
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