Metody kompenzace útlumu jasu s hloubkou obrazů získaných konfokálním mikroskopem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F06%3A00049753" target="_blank" >RIV/67985823:_____/06:00049753 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/06:12120277

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods for compensation of the light attenuation with depth of images captured by a confocal microscope

Popis výsledku v původním jazyce

A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. But images captured from deep layers of the specimen may be darker than images from the topmost layers due to light loss distortions. This effect causes difficulties in subsequent analysis of biological objects. We propose a solution using two approaches: either an online method working already during image acquisition, oran offline method assisting as a post-processing step. In the online method the gain value of a photomultiplier tube of a CLSM is controlled according to the difference of mean image intensities between the reference and currently acquired image. The offline method consists of two stages. In the first stage a standard histogram improving brightness and contrast is created from all images in the series. In the second stage image histograms are warped according to this standard histogram

Název v anglickém jazyce

Methods for compensation of the light attenuation with depth of images captured by a confocal microscope

Popis výsledku anglicky

A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. But images captured from deep layers of the specimen may be darker than images from the topmost layers due to light loss distortions. This effect causes difficulties in subsequent analysis of biological objects. We propose a solution using two approaches: either an online method working already during image acquisition, oran offline method assisting as a post-processing step. In the online method the gain value of a photomultiplier tube of a CLSM is controlled according to the difference of mean image intensities between the reference and currently acquired image. The offline method consists of two stages. In the first stage a standard histogram improving brightness and contrast is created from all images in the series. In the second stage image histograms are warped according to this standard histogram

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JC - Počítačový hardware a software

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy Research Technique

ISSN

1059-910X

e-ISSN

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Svazek periodika

69

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

624-635

Kód UT WoS článku

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EID výsledku v databázi Scopus

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