Distribution of mitochondrial nucleoids upon mitochondrial network fragmentation and network reintegration in HEPG2 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F13%3A00391723" target="_blank" >RIV/67985823:_____/13:00391723 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biocel.2012.11.019" target="_blank" >http://dx.doi.org/10.1016/j.biocel.2012.11.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biocel.2012.11.019" target="_blank" >10.1016/j.biocel.2012.11.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distribution of mitochondrial nucleoids upon mitochondrial network fragmentation and network reintegration in HEPG2 cells

Popis výsledku v původním jazyce

Mitochondrial DNA (mtDNA) is organized in nucleoids in complex with accessory proteins, proteins of mtDNA replication and gene expression machinery. A robust mtDNA genome is represented by hundreds to thousands of nucleoids in cell mitochondrion. We usedconfocal microscopy to study mitochondrial nucleoid redistribution upon mitochondrial fission and following reintegration of the mitochondrial network. Fission was induced by oxidative stress at respiration inhibition by rotenone or upon elimination ofthe protonmotive force by uncoupling or upon canceling its electrical component, delta psi(m), by valinomycin; and by silencing of mitofusin MFN2. Agent withdrawal resulted in concomitant mitochondrial network reintegration. We found two major principalmorphological states: (i) a tubular state of the mitochondrial network with equidistant nucleoid spacing, and (ii) a fragmented state of solitary spheroid objects in which several nucleoids were clustered. Reintegration of fragments into

Název v anglickém jazyce

Distribution of mitochondrial nucleoids upon mitochondrial network fragmentation and network reintegration in HEPG2 cells

Popis výsledku anglicky

Mitochondrial DNA (mtDNA) is organized in nucleoids in complex with accessory proteins, proteins of mtDNA replication and gene expression machinery. A robust mtDNA genome is represented by hundreds to thousands of nucleoids in cell mitochondrion. We usedconfocal microscopy to study mitochondrial nucleoid redistribution upon mitochondrial fission and following reintegration of the mitochondrial network. Fission was induced by oxidative stress at respiration inhibition by rotenone or upon elimination ofthe protonmotive force by uncoupling or upon canceling its electrical component, delta psi(m), by valinomycin; and by silencing of mitofusin MFN2. Agent withdrawal resulted in concomitant mitochondrial network reintegration. We found two major principalmorphological states: (i) a tubular state of the mitochondrial network with equidistant nucleoid spacing, and (ii) a fragmented state of solitary spheroid objects in which several nucleoids were clustered. Reintegration of fragments into

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biochemistry and Cell Biology

ISSN

1357-2725

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

593-603

Kód UT WoS článku

000315539900011

EID výsledku v databázi Scopus

—