Distribution of mitochondrial DNA nucleoids inside the linear tubules vs. bulk parts of mitochondrial network as visualized by 4Pi microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F15%3A00446711" target="_blank" >RIV/67985823:_____/15:00446711 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10863-015-9610-3" target="_blank" >http://dx.doi.org/10.1007/s10863-015-9610-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10863-015-9610-3" target="_blank" >10.1007/s10863-015-9610-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distribution of mitochondrial DNA nucleoids inside the linear tubules vs. bulk parts of mitochondrial network as visualized by 4Pi microscopy

Popis výsledku v původním jazyce

To visualize nucleoid distribution within the mt reticular network, we have employed three?dimensional (3D) double?color 4Pi microscopy. The mt network was visualized in hepatocellular carcinoma HepG2 cells via mt?matrix?addressed GFP, while 3D immunocytochemistry of mtSSB was performed. Optimization of iso-surface computation threshold for nucleoid 4Pi images to 30 led to an average nucleoid diameter of 219110 nm and 224100 nm in glucose? and galactose?cultivated HepG2 cells (the latter with obligatoryoxidative phosphorylation). We have positioned mtDNA nucleoids within the mt reticulum network and refined our model for nucleoid redistribution within the fragmented network ?? clustering of up to 10 nucleoids in 2 m diameter mitochondrial spheroids ofa fragmented mt network, arising from an original 10 m mt tubule of a 400 nm diameter. However, the theoretically fragmented bulk parts were observed most frequently as being reintegrated into the continuous mt network in 4Pi images. Sin

Název v anglickém jazyce

Distribution of mitochondrial DNA nucleoids inside the linear tubules vs. bulk parts of mitochondrial network as visualized by 4Pi microscopy

Popis výsledku anglicky

To visualize nucleoid distribution within the mt reticular network, we have employed three?dimensional (3D) double?color 4Pi microscopy. The mt network was visualized in hepatocellular carcinoma HepG2 cells via mt?matrix?addressed GFP, while 3D immunocytochemistry of mtSSB was performed. Optimization of iso-surface computation threshold for nucleoid 4Pi images to 30 led to an average nucleoid diameter of 219110 nm and 224100 nm in glucose? and galactose?cultivated HepG2 cells (the latter with obligatoryoxidative phosphorylation). We have positioned mtDNA nucleoids within the mt reticulum network and refined our model for nucleoid redistribution within the fragmented network ?? clustering of up to 10 nucleoids in 2 m diameter mitochondrial spheroids ofa fragmented mt network, arising from an original 10 m mt tubule of a 400 nm diameter. However, the theoretically fragmented bulk parts were observed most frequently as being reintegrated into the continuous mt network in 4Pi images. Sin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Bioenergetics and Biomembranes

ISSN

0145-479X

e-ISSN

—

Svazek periodika

47

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

255-263

Kód UT WoS článku

000354208400008

EID výsledku v databázi Scopus

2-s2.0-84937765227