Oxidativní fosforylace a energetický status jsou reflektovány morfologií mitochondriální sítě u INS-1E a HEP-G2 buněk zobrazených 4Pi mikroskopií

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F08%3A00314590" target="_blank" >RIV/67985823:_____/08:00314590 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy

Popis výsledku v původním jazyce

Using 4Pi microscopy, 3D morphology changes of mitochondrial reticulum network responding to decreased oxidative phosphorylation have been observed in INS-1E and HEP-G2 cells at an unprecedented 100 nm level of detail. In INS-1E cells a single interconnected mitochondrion with a nearly constant 262+26 nm tubule diameter was found. With rotenone inhibiting respiration to 10%, disintegration into several reticula and numerous 300 nm spheres or short tubules was observed. De-energization by uncoupling additionally led to formation of rings and bulky cisternae of 1.4+0.4 ?m diameter. Rotenone and uncoupler acted synergically in INS-1E cells and increased fusion (ongoing with fission) forming bowl-like shapes. In HEP-G2 cells fission partially ceased with FCCP plus rotenone. Thus we have revealed previously undescribed details for shapes upon mitochondrial disintegration and clearly demonstrate that high resolution 3D microscopy is required for visualization of mitochondrial network

Název v anglickém jazyce

Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy

Popis výsledku anglicky

Using 4Pi microscopy, 3D morphology changes of mitochondrial reticulum network responding to decreased oxidative phosphorylation have been observed in INS-1E and HEP-G2 cells at an unprecedented 100 nm level of detail. In INS-1E cells a single interconnected mitochondrion with a nearly constant 262+26 nm tubule diameter was found. With rotenone inhibiting respiration to 10%, disintegration into several reticula and numerous 300 nm spheres or short tubules was observed. De-energization by uncoupling additionally led to formation of rings and bulky cisternae of 1.4+0.4 ?m diameter. Rotenone and uncoupler acted synergically in INS-1E cells and increased fusion (ongoing with fission) forming bowl-like shapes. In HEP-G2 cells fission partially ceased with FCCP plus rotenone. Thus we have revealed previously undescribed details for shapes upon mitochondrial disintegration and clearly demonstrate that high resolution 3D microscopy is required for visualization of mitochondrial network

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Bioenergetics

ISSN

0005-2728

e-ISSN

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Svazek periodika

1777

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

257696400039

EID výsledku v databázi Scopus

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