High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00461653" target="_blank" >RIV/67985823:_____/16:00461653 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jbiotec.2016.06.023" target="_blank" >http://dx.doi.org/10.1016/j.jbiotec.2016.06.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jbiotec.2016.06.023" target="_blank" >10.1016/j.jbiotec.2016.06.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species

Popis výsledku v původním jazyce

New antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fluorescence method suitable for identifying new potent drugs that directly target yeast cell surface structures, causing cell permeabilization and thus bypassing the multidrug resistance mechanisms of pathogens. The fluorescence assay enabled us to detect with high sensitivity damage to the Candida plasma membrane (its hyperpolarization and permeabilization) as a result of short-term exposure to the antifungal compounds. Results can be obtained in 1–2 h with minimal effort and consumption of the tested compounds, also 96 samples can be analysed simultaneously. We used this method to study antimicrobial peptides isolated from the venom of bees and their synthetic analogs, compare the potency of the peptides and determine their minimal effective concentrations. The antimicrobial peptides were able to kill yeast cells at low concentrations within a 15-min treatment, the LL-III peptide exhibited a broad spectrum of antifungal activity on various Saccharomyces, pathogenic Candida and osmotolerant yeast species.

Název v anglickém jazyce

High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species

Popis výsledku anglicky

New antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fluorescence method suitable for identifying new potent drugs that directly target yeast cell surface structures, causing cell permeabilization and thus bypassing the multidrug resistance mechanisms of pathogens. The fluorescence assay enabled us to detect with high sensitivity damage to the Candida plasma membrane (its hyperpolarization and permeabilization) as a result of short-term exposure to the antifungal compounds. Results can be obtained in 1–2 h with minimal effort and consumption of the tested compounds, also 96 samples can be analysed simultaneously. We used this method to study antimicrobial peptides isolated from the venom of bees and their synthetic analogs, compare the potency of the peptides and determine their minimal effective concentrations. The antimicrobial peptides were able to kill yeast cells at low concentrations within a 15-min treatment, the LL-III peptide exhibited a broad spectrum of antifungal activity on various Saccharomyces, pathogenic Candida and osmotolerant yeast species.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biotechnology

ISSN

0168-1656

e-ISSN

—

Svazek periodika

233

Číslo periodika v rámci svazku

Sep 10

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

26-33

Kód UT WoS článku

000380819300004

EID výsledku v databázi Scopus

2-s2.0-84978253165