Lipophilic phosphorescent gold(I) clusters as selective probes for visualization of lipid droplets by two-photon microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00481073" target="_blank" >RIV/67985823:_____/17:00481073 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jphotochem.2016.08.017" target="_blank" >http://dx.doi.org/10.1016/j.jphotochem.2016.08.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jphotochem.2016.08.017" target="_blank" >10.1016/j.jphotochem.2016.08.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipophilic phosphorescent gold(I) clusters as selective probes for visualization of lipid droplets by two-photon microscopy

Popis výsledku v původním jazyce

The development of organelle-specific phosphorescent multiphoton probes is a rapidly growing area in biomedical imaging. Phosphorescent properties allow spectral or time-resolved discrimination from autofluorescence, while the use of two-photon microscopy provides higher spatial resolution as well as deeper penetration into the sample compared to conventional microscopies. The main challenge in this area is the design of probes that combine a complex of desired properties. Here we present investigation of the phosphorescent homoleptic alkynyl gold(I) cluster, (AuC2R)(10) (R 2,6-dimethyl-4-heptanol), which combines nonlinear optical properties with selectivity to lipid droplets of adipocytes and other cells. This label reveals high two-photon absorption and emission cross-sections in hexane, good solubility in triglycerides (vegetable oil) and microsecond lifetimes both in solution and in biological tissues. These features make it a promising probe for multiphoton bioimaging as demonstrated by successful visualization of various adipose tissues (subcutaneous fat, visceral adipose tissue, adipose tissue of mesentery) of different animals (pigeon, chicken, mouse) as well as lipid droplets in Hep G2 cells. The complex demonstrates high selectivity to lipid droplets in animal tissues and cells. The probe lifetimes in microsecond domain made possible its visualization using phosphorescent lifetime imaging (PLIM) techniques.

Název v anglickém jazyce

Lipophilic phosphorescent gold(I) clusters as selective probes for visualization of lipid droplets by two-photon microscopy

Popis výsledku anglicky

The development of organelle-specific phosphorescent multiphoton probes is a rapidly growing area in biomedical imaging. Phosphorescent properties allow spectral or time-resolved discrimination from autofluorescence, while the use of two-photon microscopy provides higher spatial resolution as well as deeper penetration into the sample compared to conventional microscopies. The main challenge in this area is the design of probes that combine a complex of desired properties. Here we present investigation of the phosphorescent homoleptic alkynyl gold(I) cluster, (AuC2R)(10) (R 2,6-dimethyl-4-heptanol), which combines nonlinear optical properties with selectivity to lipid droplets of adipocytes and other cells. This label reveals high two-photon absorption and emission cross-sections in hexane, good solubility in triglycerides (vegetable oil) and microsecond lifetimes both in solution and in biological tissues. These features make it a promising probe for multiphoton bioimaging as demonstrated by successful visualization of various adipose tissues (subcutaneous fat, visceral adipose tissue, adipose tissue of mesentery) of different animals (pigeon, chicken, mouse) as well as lipid droplets in Hep G2 cells. The complex demonstrates high selectivity to lipid droplets in animal tissues and cells. The probe lifetimes in microsecond domain made possible its visualization using phosphorescent lifetime imaging (PLIM) techniques.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

<a href="/cs/project/LM2015062" target="_blank" >LM2015062: Národní infrastruktura pro biologické a medicínské zobrazování</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Photochemistry and Photobiology A-Chemistry

ISSN

1010-6030

e-ISSN

—

Svazek periodika

332

Číslo periodika v rámci svazku

Jan 1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

122-130

Kód UT WoS článku

000387837300014

EID výsledku v databázi Scopus

2-s2.0-84983444485