Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F18%3A00489869" target="_blank" >RIV/67985823:_____/18:00489869 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.matlet.2017.12.004" target="_blank" >http://dx.doi.org/10.1016/j.matlet.2017.12.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.matlet.2017.12.004" target="_blank" >10.1016/j.matlet.2017.12.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Popis výsledku v původním jazyce

Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

Název v anglickém jazyce

Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Popis výsledku anglicky

Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Projekt

<a href="/cs/project/NV15-33018A" target="_blank" >NV15-33018A: Využití kmenových buněk tukové tkáně získané liposukcí v tkáňovém inženýrství</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Letters

ISSN

0167-577X

e-ISSN

—

Svazek periodika

214

Číslo periodika v rámci svazku

Mar 1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

186-189

Kód UT WoS článku

000419724400048

EID výsledku v databázi Scopus

2-s2.0-85042236906