Optical Mapping Can Reveal Two-Dimensional Morphology of Atrioventricular Insulation in the Foetal Avian Heart

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F18%3A00501676" target="_blank" >RIV/67985823:_____/18:00501676 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/18:PU129627

Výsledek na webu

<a href="http://www.cinc.org/cinc-papers-on-line/" target="_blank" >http://www.cinc.org/cinc-papers-on-line/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22489/CinC.2018.055" target="_blank" >10.22489/CinC.2018.055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optical Mapping Can Reveal Two-Dimensional Morphology of Atrioventricular Insulation in the Foetal Avian Heart

Popis výsledku v původním jazyce

Syndromes of ventricular pre-excitation or atrio-ventricular (AV) re-entry are believed to results from inappropriate formation of the AV junction (AVJ) during prenatal development. However, technical limitations pose challenge to study foetal heart electrophysiology and are hampering understanding formation of accessory pathways (APs) bypassing AVJ. Here, we present a novel approach of retrograde perfusion for optical mapping of chicken foetal heart. Together with embryonic heart mapping, we were able to investigate AVJ electrical remodelling during the entire prenatal period. AV electrical insulation was observed concomitant to functional deployment of the conduction system at the time of ventricular septation. However, persistence of ectopic AV electrical continuity was still detected in post-septation foetal hearts. APs registered in 40% of prenatal day 13 (localised in the central anterior or left posterior side) were progressively resolved and none were detected on prenatal day 18. The optical mapping approach thus proved its ability to track AVJ electrical remodelling during whole prenatal chicken development and spatiotemporally localise electrically active APs.

Název v anglickém jazyce

Optical Mapping Can Reveal Two-Dimensional Morphology of Atrioventricular Insulation in the Foetal Avian Heart

Popis výsledku anglicky

Syndromes of ventricular pre-excitation or atrio-ventricular (AV) re-entry are believed to results from inappropriate formation of the AV junction (AVJ) during prenatal development. However, technical limitations pose challenge to study foetal heart electrophysiology and are hampering understanding formation of accessory pathways (APs) bypassing AVJ. Here, we present a novel approach of retrograde perfusion for optical mapping of chicken foetal heart. Together with embryonic heart mapping, we were able to investigate AVJ electrical remodelling during the entire prenatal period. AV electrical insulation was observed concomitant to functional deployment of the conduction system at the time of ventricular septation. However, persistence of ectopic AV electrical continuity was still detected in post-septation foetal hearts. APs registered in 40% of prenatal day 13 (localised in the central anterior or left posterior side) were progressively resolved and none were detected on prenatal day 18. The optical mapping approach thus proved its ability to track AVJ electrical remodelling during whole prenatal chicken development and spatiotemporally localise electrically active APs.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Computing in Cardiology 2018

ISBN

—

ISSN

2325-887X

e-ISSN

—

Počet stran výsledku

4

Strana od-do

—

Název nakladatele

IEEE

Místo vydání

New York

Místo konání akce

Maastricht

Datum konání akce

23. 9. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000482598700281