Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00524475" target="_blank" >RIV/67985823:_____/20:00524475 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/20:10419299

Výsledek na webu

<a href="https://doi.org/10.1016/j.apsusc.2020.146128" target="_blank" >https://doi.org/10.1016/j.apsusc.2020.146128</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apsusc.2020.146128" target="_blank" >10.1016/j.apsusc.2020.146128</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE)

Popis výsledku v původním jazyce

Covalent immobilization of biomolecules to surfaces is desirable in applications in biomedicine and biotechnology, such as biosensors, protein microarrays and implantable biomedical devices. Surface-embedded radicals in polymers, produced by plasma immersion ion implantation, are known to covalently immobilize biomolecules directly from buffer without additional reagents. Here we explore the prospects for creating a surface activated for direct covalent immobilization using a dielectric barrier discharge in air at atmospheric pressure, eliminating the need for vacuum and gas feed systems. We find that a surface activation process at atmospheric pressure in air can activate polytetrafluoroethylene (PTFE) in order to achieve reagent-free covalent immobilization of biomolecules. The presence of surface immobilized protein was verified by X-ray photoelectron spectroscopy (XPS), and its covalent immobilization was demonstrated by resilience to rigorous SDS washing at elevated temperature. Time course immobilization studies show that the covalent coupling capability of the activated surfaces is retained for several days. Proof-of-concept cell assays with immobilized tropoelastin demonstrate the technique’s ability to present functional cell binding molecules for the production of truly bioactive surfaces.

Název v anglickém jazyce

Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE)

Popis výsledku anglicky

Covalent immobilization of biomolecules to surfaces is desirable in applications in biomedicine and biotechnology, such as biosensors, protein microarrays and implantable biomedical devices. Surface-embedded radicals in polymers, produced by plasma immersion ion implantation, are known to covalently immobilize biomolecules directly from buffer without additional reagents. Here we explore the prospects for creating a surface activated for direct covalent immobilization using a dielectric barrier discharge in air at atmospheric pressure, eliminating the need for vacuum and gas feed systems. We find that a surface activation process at atmospheric pressure in air can activate polytetrafluoroethylene (PTFE) in order to achieve reagent-free covalent immobilization of biomolecules. The presence of surface immobilized protein was verified by X-ray photoelectron spectroscopy (XPS), and its covalent immobilization was demonstrated by resilience to rigorous SDS washing at elevated temperature. Time course immobilization studies show that the covalent coupling capability of the activated surfaces is retained for several days. Proof-of-concept cell assays with immobilized tropoelastin demonstrate the technique’s ability to present functional cell binding molecules for the production of truly bioactive surfaces.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Surface Science

ISSN

0169-4332

e-ISSN

—

Svazek periodika

518

Číslo periodika v rámci svazku

Jul 15

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

146128

Kód UT WoS článku

000531746300005

EID výsledku v databázi Scopus

2-s2.0-85082704998