Cellulases and their promising use in tissue engineering and cell sheet technology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00636207" target="_blank" >RIV/67985823:_____/25:00636207 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/25:00636207 RIV/60461373:22310/25:43933102

Výsledek na webu

<a href="https://doi.org/10.1016/j.matdes.2025.114111" target="_blank" >https://doi.org/10.1016/j.matdes.2025.114111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.matdes.2025.114111" target="_blank" >10.1016/j.matdes.2025.114111</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cellulases and their promising use in tissue engineering and cell sheet technology

Popis výsledku v původním jazyce

Bacterial nanocellulose (BNC) was used to generate continuous scaffold-free sheets of human keratinocytes (HaCaT) after degradation by cellulase from Trichoderma reesei. First, we successfully grafted acidic cellulase from Trichoderma reesei onto a BNC nanostructure and prepared degradable BNC that dissolved in phosphate-buffered saline within one week. In addition, we selected a cellulase solution that degrades BNC within three hours under physiological conditions. HaCaTs were cultured on four different BNC samples: air-dried (BNC_AD), lyophilized (BNC_L) and plasma-modified (PM) BNC exposed to a direct current Ar+plasma discharge (BNC_AD_PM and BNC_L_PM). On days 1 and 3, the cells were stained with the LIVE/DEAD viability/cytotoxicity kit. The significantly highest cell number on day 3 was found on the BNC_AD_PM membrane (median 65,025 cells/cm2), similar to the standard cell culture polystyrene (median 58,553 cells/cm2). Cell viability on day 3 was greater than 90 % for all samples except BNC_L (62 %). Cell island formation was also preferentially observed on BNC_AD_PM samples. The significant positive effect of PM on cell growth was shown after BNC degradation on day 10 after cell seeding, when continuous sheets of viable keratinocytes were separated from the BNC samples by exposure to a cellulase solution (37.5 U/ml, pH 6.5) for 3 h. The number of cells obtained by cultivation on BNC_PM greatly exceeded the number of cells obtained from unmodified BNC. The approaches presented here may be useful for the preparation of (1) degradable BNC scaffolds for tissue engineering and (2) continuous scaffold-free cell sheets applicable in wound healing.

Název v anglickém jazyce

Cellulases and their promising use in tissue engineering and cell sheet technology

Popis výsledku anglicky

Bacterial nanocellulose (BNC) was used to generate continuous scaffold-free sheets of human keratinocytes (HaCaT) after degradation by cellulase from Trichoderma reesei. First, we successfully grafted acidic cellulase from Trichoderma reesei onto a BNC nanostructure and prepared degradable BNC that dissolved in phosphate-buffered saline within one week. In addition, we selected a cellulase solution that degrades BNC within three hours under physiological conditions. HaCaTs were cultured on four different BNC samples: air-dried (BNC_AD), lyophilized (BNC_L) and plasma-modified (PM) BNC exposed to a direct current Ar+plasma discharge (BNC_AD_PM and BNC_L_PM). On days 1 and 3, the cells were stained with the LIVE/DEAD viability/cytotoxicity kit. The significantly highest cell number on day 3 was found on the BNC_AD_PM membrane (median 65,025 cells/cm2), similar to the standard cell culture polystyrene (median 58,553 cells/cm2). Cell viability on day 3 was greater than 90 % for all samples except BNC_L (62 %). Cell island formation was also preferentially observed on BNC_AD_PM samples. The significant positive effect of PM on cell growth was shown after BNC degradation on day 10 after cell seeding, when continuous sheets of viable keratinocytes were separated from the BNC samples by exposure to a cellulase solution (37.5 U/ml, pH 6.5) for 3 h. The number of cells obtained by cultivation on BNC_PM greatly exceeded the number of cells obtained from unmodified BNC. The approaches presented here may be useful for the preparation of (1) degradable BNC scaffolds for tissue engineering and (2) continuous scaffold-free cell sheets applicable in wound healing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials and Design

ISSN

0264-1275

e-ISSN

1873-4197

Svazek periodika

254

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

114111

Kód UT WoS článku

001502161100002

EID výsledku v databázi Scopus

2-s2.0-105006742249