Functional analysis of two genes coding for distinct cation diffusion facilitators of the cadmium-accumulating fungus Agaricus crocodilinus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985831%3A_____%2F25%3A00617167" target="_blank" >RIV/67985831:_____/25:00617167 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/25:00617718 RIV/60461373:22330/25:43931634 RIV/60461373:22810/25:43931634

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1878614625000169?dgcid=coauthor" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1878614625000169?dgcid=coauthor</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.funbio.2025.101550" target="_blank" >10.1016/j.funbio.2025.101550</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Functional analysis of two genes coding for distinct cation diffusion facilitators of the cadmium-accumulating fungus Agaricus crocodilinus

Popis výsledku v původním jazyce

The gilled mushroom Agaricus crocodilinus (Agaricaceae) analyzed in this study hyperaccumulated Cd and showed common Zn and very low Mn concentrations. To gain an insight into the handling of heavy metals in this saprotrophic species, its two genes of the cation diffusion facilitator (CDF) protein family were isolated, AciCDF1 and AciDF2, encoding the membrane transporters of the Zn-CDF and Mn-CDF subfamilies, respectively. When expressed in the model, metal-sensitive yeast, AcCDF1 conferred marked Zn tolerance and promoted the intracellular accumulation of Zn. Green fluorescent protein (GFP) tagging of AcCDF1 visualized the functional protein predominantly in the tonoplast, indicating that AcCDF1 can mediate the transport of Zn into vacuoles, which are used for deposition of excess Zn in most fungi. AcCDF2 conferred a high degree of Mn tolerance to model yeast, in which the transport-active AcCDF2:GFP fusion was localized to the plasma membrane, suggesting a role in Mn export and thus reduced Mn accumulation. Furthermore, the AcCDF2 gene appeared to be Mn-inducible in A. crocodilinus, suggesting an Mn efflux function of AcCDF2. Neither AcCDFs nor the mutant AcCDF1 variants constructed to mimic transmembrane tetrahedral Cd transport sites manifested appreciable Cd-related phenotypes in yeast models, and further efforts are needed to elucidate the mechanism underlying Cd hyperaccumulation in A. crocodilinus.

Název v anglickém jazyce

Functional analysis of two genes coding for distinct cation diffusion facilitators of the cadmium-accumulating fungus Agaricus crocodilinus

Popis výsledku anglicky

The gilled mushroom Agaricus crocodilinus (Agaricaceae) analyzed in this study hyperaccumulated Cd and showed common Zn and very low Mn concentrations. To gain an insight into the handling of heavy metals in this saprotrophic species, its two genes of the cation diffusion facilitator (CDF) protein family were isolated, AciCDF1 and AciDF2, encoding the membrane transporters of the Zn-CDF and Mn-CDF subfamilies, respectively. When expressed in the model, metal-sensitive yeast, AcCDF1 conferred marked Zn tolerance and promoted the intracellular accumulation of Zn. Green fluorescent protein (GFP) tagging of AcCDF1 visualized the functional protein predominantly in the tonoplast, indicating that AcCDF1 can mediate the transport of Zn into vacuoles, which are used for deposition of excess Zn in most fungi. AcCDF2 conferred a high degree of Mn tolerance to model yeast, in which the transport-active AcCDF2:GFP fusion was localized to the plasma membrane, suggesting a role in Mn export and thus reduced Mn accumulation. Furthermore, the AcCDF2 gene appeared to be Mn-inducible in A. crocodilinus, suggesting an Mn efflux function of AcCDF2. Neither AcCDFs nor the mutant AcCDF1 variants constructed to mimic transmembrane tetrahedral Cd transport sites manifested appreciable Cd-related phenotypes in yeast models, and further efforts are needed to elucidate the mechanism underlying Cd hyperaccumulation in A. crocodilinus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/GA19-06759S" target="_blank" >GA19-06759S: Hyperakumulace kadmia ve velkých houbách: od izotopů k proteinům a bakteriálním komunitám</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fungal Biology

ISSN

1878-6146

e-ISSN

1878-6162

Svazek periodika

129

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

101550

Kód UT WoS článku

001428437300001

EID výsledku v databázi Scopus

2-s2.0-85217981252