Characterization of pbt genes conferring increased Pb(2+) and Cd(2+) tolerance upon Achromobacter xylosoxidans A8

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00422943" target="_blank" >RIV/68378050:_____/13:00422943 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.resmic.2013.10.002" target="_blank" >http://dx.doi.org/10.1016/j.resmic.2013.10.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.resmic.2013.10.002" target="_blank" >10.1016/j.resmic.2013.10.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of pbt genes conferring increased Pb(2+) and Cd(2+) tolerance upon Achromobacter xylosoxidans A8

Popis výsledku v původním jazyce

The cluster of pbtTFYRABC genes is carried by plasmid pA81. Its elimination from Achromobacter xylosoxidans A8 resulted in increased sensitivity towards Pb(2+) and Cd(2+). Predicted pbtTRABC products share strong similarities with Pb(2+) uptake transporter PbrT, transcriptional regulator PbrR, metal efflux P1-ATPases PbrA and CadA, undecaprenyl pyrophosphatase PbrB and its signal peptidase PbrC from Cupriavidus metallidurans CH34. Expression of pbtABC or pbtA in a metal-sensitive Escherichia coli GG48 rendered the strain Pb(2+)-, Cd(2+)- and Zn(2+)-tolerant and caused decreased accumulation of the metal ions. Accumulation of Pb(2+), but not of Cd(2+) or Zn(2+), was promoted in E. coli expressing pbtT. Additional genes of the pbt cluster are pbtF and pbtY, which encode the cation diffusion facilitator (CDF)-like transporter and a putative fatty acid hydroxylase of unknown function, respectively. Expression of pbtF did not confer increased metal tolerance upon E. coli GG48, although the

Název v anglickém jazyce

Characterization of pbt genes conferring increased Pb(2+) and Cd(2+) tolerance upon Achromobacter xylosoxidans A8

Popis výsledku anglicky

The cluster of pbtTFYRABC genes is carried by plasmid pA81. Its elimination from Achromobacter xylosoxidans A8 resulted in increased sensitivity towards Pb(2+) and Cd(2+). Predicted pbtTRABC products share strong similarities with Pb(2+) uptake transporter PbrT, transcriptional regulator PbrR, metal efflux P1-ATPases PbrA and CadA, undecaprenyl pyrophosphatase PbrB and its signal peptidase PbrC from Cupriavidus metallidurans CH34. Expression of pbtABC or pbtA in a metal-sensitive Escherichia coli GG48 rendered the strain Pb(2+)-, Cd(2+)- and Zn(2+)-tolerant and caused decreased accumulation of the metal ions. Accumulation of Pb(2+), but not of Cd(2+) or Zn(2+), was promoted in E. coli expressing pbtT. Additional genes of the pbt cluster are pbtF and pbtY, which encode the cation diffusion facilitator (CDF)-like transporter and a putative fatty acid hydroxylase of unknown function, respectively. Expression of pbtF did not confer increased metal tolerance upon E. coli GG48, although the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0520" target="_blank" >1M0520: Centrum aplikované genomiky</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in microbiology

ISSN

1769-7123

e-ISSN

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Svazek periodika

164

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

10

Strana od-do

1009-1018

Kód UT WoS článku

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EID výsledku v databázi Scopus

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