Optické vláknové biosenzory využívající enzymatického nosiče pro monitorování glukózy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F07%3A00091561" target="_blank" >RIV/67985858:_____/07:00091561 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985882:_____/07:00091561

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Optical Fibre Biosensors Using Enzymatic Transducers to Monitor Glucose

Popis výsledku v původním jazyce

The construction and performance of a novel enzyme based optical sensor for in situ continuous monitoring of glucose in biotechnological production processes is presented. Sensitive optical coatings are formed from inorganic?organic hybrid polymers (ORMOCER) combined with a flurophore (Ru complex) and an enzyme, and applied to lenses, declad polymer optical fibre and polymer clad silica fibre. The enzyme, glucose oxidase, catalyzes oxidization of glucose to gluconic acid by depleting oxygen. Oxygen consumption is determined by measuring the fluorescence lifetime of metal Ru complexes which are quenched by oxygen. The coatings developed were designed to adhere to glass and polymer surfaces, to be compatible with enzymes and Ru complexes, and were demonstrated both as double- and single-layer structures. The sensor response to gaseous oxygen, dissolved oxygen and dissolved glucose was measured via fluorescence lifetime changes.

Název v anglickém jazyce

Optical Fibre Biosensors Using Enzymatic Transducers to Monitor Glucose

Popis výsledku anglicky

The construction and performance of a novel enzyme based optical sensor for in situ continuous monitoring of glucose in biotechnological production processes is presented. Sensitive optical coatings are formed from inorganic?organic hybrid polymers (ORMOCER) combined with a flurophore (Ru complex) and an enzyme, and applied to lenses, declad polymer optical fibre and polymer clad silica fibre. The enzyme, glucose oxidase, catalyzes oxidization of glucose to gluconic acid by depleting oxygen. Oxygen consumption is determined by measuring the fluorescence lifetime of metal Ru complexes which are quenched by oxygen. The coatings developed were designed to adhere to glass and polymer surfaces, to be compatible with enzymes and Ru complexes, and were demonstrated both as double- and single-layer structures. The sensor response to gaseous oxygen, dissolved oxygen and dissolved glucose was measured via fluorescence lifetime changes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>R - Projekt Ramcoveho programu EK

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Measurement Science and Technology

ISSN

0957-0233

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

3177-3186

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—