A Biosensor with Encapsulated Bioreporters-Optical Fiber Element for Enhanced Detection of Bioluminescence.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F18%3A00490436" target="_blank" >RIV/67985858:_____/18:00490436 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >http://dx.doi.org/10.1088/1361-6501/aac633</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >10.1088/1361-6501/aac633</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Biosensor with Encapsulated Bioreporters-Optical Fiber Element for Enhanced Detection of Bioluminescence.

Popis výsledku v původním jazyce

The whole-cell optical fiber sensor is proposed as a real in-situ detector for on-line monitoring of the environmental pollution in remote localities. This sensor needs an optimal design of an optical fiber element for ensure effective coupling of low intense light produced by bioluminescent bioreporters. We calculated that only 3.5% of all photons, produced by one bacterium placed on the front end of the optical fiber - polymer cladded silica fiber, are coupled and guided. Up to six times higher intensity of detected light was calculated by enlargement of an area covered with bacteria using optical fiber element with maximal transmittance. The results were confirmed by measurement with immobilized Pseudomonas fluorescens HK44. The influence of physical properties and optical fiber geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.

Název v anglickém jazyce

A Biosensor with Encapsulated Bioreporters-Optical Fiber Element for Enhanced Detection of Bioluminescence.

Popis výsledku anglicky

The whole-cell optical fiber sensor is proposed as a real in-situ detector for on-line monitoring of the environmental pollution in remote localities. This sensor needs an optimal design of an optical fiber element for ensure effective coupling of low intense light produced by bioluminescent bioreporters. We calculated that only 3.5% of all photons, produced by one bacterium placed on the front end of the optical fiber - polymer cladded silica fiber, are coupled and guided. Up to six times higher intensity of detected light was calculated by enlargement of an area covered with bacteria using optical fiber element with maximal transmittance. The results were confirmed by measurement with immobilized Pseudomonas fluorescens HK44. The influence of physical properties and optical fiber geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Measurement Science and Technology

ISSN

0957-0233

e-ISSN

—

Svazek periodika

29

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

—

Kód UT WoS článku

000435378000001

EID výsledku v databázi Scopus

2-s2.0-85048984380