A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00321984" target="_blank" >RIV/68407700:21460/18:00321984 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >http://dx.doi.org/10.1088/1361-6501/aac633</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >10.1088/1361-6501/aac633</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence

Popis výsledku v původním jazyce

It is proposed that a whole-cell optical fiber biosensor is used as an in situ first warning detector sensor for online monitoring of environmental pollution (e.g. waste water) in remote localities. The base requirement for this sensor is a very high sensitivity topollution. The work shows a simple way to increase sensor sensitivity, optimizing the shape of an optical fiber element (OFE) for more effective coupling of the low intensity light produced by bioluminescent bioreporters. Theoretical calculations of coupling efficiency showed that only 3.5% of photons produced by one bacterium placed on the front end of polymer-clad silica (PCS) fiber is guided to the detector. Our designed tapered OFEs with immobilized bioluminescent bioreporters Pseudomonas fluorescens HK44, with maximal transmittance, led to a sixfold increase in detected bioluminescence compared to that of the PCS fiber with the same active layer. It is shown that the shape of the OFE determines its transmission. The influences of physical properties and OFE geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.

Název v anglickém jazyce

A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence

Popis výsledku anglicky

It is proposed that a whole-cell optical fiber biosensor is used as an in situ first warning detector sensor for online monitoring of environmental pollution (e.g. waste water) in remote localities. The base requirement for this sensor is a very high sensitivity topollution. The work shows a simple way to increase sensor sensitivity, optimizing the shape of an optical fiber element (OFE) for more effective coupling of the low intensity light produced by bioluminescent bioreporters. Theoretical calculations of coupling efficiency showed that only 3.5% of photons produced by one bacterium placed on the front end of polymer-clad silica (PCS) fiber is guided to the detector. Our designed tapered OFEs with immobilized bioluminescent bioreporters Pseudomonas fluorescens HK44, with maximal transmittance, led to a sixfold increase in detected bioluminescence compared to that of the PCS fiber with the same active layer. It is shown that the shape of the OFE determines its transmission. The influences of physical properties and OFE geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20201 - Electrical and electronic engineering

Projekt

<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Measurement Science and Technology

ISSN

0957-0233

e-ISSN

1361-6501

Svazek periodika

29

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000435378000001

EID výsledku v databázi Scopus

2-s2.0-85048984380