Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202769" target="_blank" >RIV/68407700:21460/12:00202769 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence

Popis výsledku v původním jazyce

The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence(BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by thegenetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shapefrom point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was

Název v anglickém jazyce

Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence

Popis výsledku anglicky

The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence(BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by thegenetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shapefrom point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was

Druh

D - Stať ve sborníku

CEP obor

JB - Senzory, čidla, měření a regulace

OECD FORD obor

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Projekt

<a href="/cs/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: Rozvoj výzkumného týmu BIO-OPT-XUV na FBMI ČVUT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Instuments and Methods for Biology and Medicine 2012

ISBN

978-80-01-05119-1

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

49-51

Název nakladatele

Czech Technical University

Místo vydání

Prague

Místo konání akce

Kladno

Datum konání akce

29. 5. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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