Whole-Cell Detectors of Aromatic Hydrocarbon Contaminants Constructed by Immobilization of Bioreporters on Special Optical Fiber Elements

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Whole-Cell Detectors of Aromatic Hydrocarbon Contaminants Constructed by Immobilization of Bioreporters on Special Optical Fiber Elements

Popis výsledku v původním jazyce

Whole-cell fiber-optic sensor of toluene was prepared by adsorption of Pseudomonas putida TVA8 bioluminescent bioreporter onto a tapered optical fiber element (OFE). Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane treatment of the OFE. Repeatability of preparation and response of the active part of biosensor was demonstrated in five trials with different OFE geometries, possessing transmittances up to 5.0%, in which the bioluminescence was induced with toluene solution (26.5 mg L-1) for the period of 2 weeks. Longevity of inductions of the biosensor was tested in over 135 days trial, in which the biosensor was induced 68 times with toluene solution (26.5 mg L-1 ). The intensities of bioluminescence gradually decreased due to release of the adsorbed cells but were significantly higher than background noise of the detector. The intensities of bioluminescence induced with contaminated ground water were lower than in mineral medium with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor layered with positively induced P. putida TVA8 bioreporters was a reliable detector of toluene. Further experiments also immobilized constitutively bioluminescent cells of Escherichia coli 652T7 on OFEs with polyethyleneimine, repetitively induced with Lauria-Bertani medium.

Název v anglickém jazyce

Whole-Cell Detectors of Aromatic Hydrocarbon Contaminants Constructed by Immobilization of Bioreporters on Special Optical Fiber Elements

Popis výsledku anglicky

Whole-cell fiber-optic sensor of toluene was prepared by adsorption of Pseudomonas putida TVA8 bioluminescent bioreporter onto a tapered optical fiber element (OFE). Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane treatment of the OFE. Repeatability of preparation and response of the active part of biosensor was demonstrated in five trials with different OFE geometries, possessing transmittances up to 5.0%, in which the bioluminescence was induced with toluene solution (26.5 mg L-1) for the period of 2 weeks. Longevity of inductions of the biosensor was tested in over 135 days trial, in which the biosensor was induced 68 times with toluene solution (26.5 mg L-1 ). The intensities of bioluminescence gradually decreased due to release of the adsorbed cells but were significantly higher than background noise of the detector. The intensities of bioluminescence induced with contaminated ground water were lower than in mineral medium with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor layered with positively induced P. putida TVA8 bioreporters was a reliable detector of toluene. Further experiments also immobilized constitutively bioluminescent cells of Escherichia coli 652T7 on OFEs with polyethyleneimine, repetitively induced with Lauria-Bertani medium.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Instruments and Methods for Biology and Medicine 2020

ISBN

9788001067963

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

10-16

Název nakladatele

České vysoké učení technické v Praze

Místo vydání

Praha

Místo konání akce

Kladno

Datum konání akce

8. 10. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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