Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F19%3A00547305" target="_blank" >RIV/67985858:_____/19:00547305 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/19:00336443

Výsledek na webu

<a href="https://ieeexplore.ieee.org/document/8940080" target="_blank" >https://ieeexplore.ieee.org/document/8940080</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/SENSORSNANO44414.2019.8940080" target="_blank" >10.1109/SENSORSNANO44414.2019.8940080</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements.

Popis výsledku v původním jazyce

A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L-1) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.

Název v anglickém jazyce

Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements.

Popis výsledku anglicky

A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L-1) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

International Conference on Sensors and Nanotechnology IEEE /1./

ISBN

978-1-5386-5619-8

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

185-188

Název nakladatele

Institute of Electrical and Electronics Engineers Inc.

Místo vydání

New York

Místo konání akce

Penang

Datum konání akce

24. 7. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000525734000047