Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F19%3A00336443" target="_blank" >RIV/68407700:21460/19:00336443 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985858:_____/19:00547305

Výsledek na webu

<a href="http://dx.doi.org/10.1109/SENSORSNANO44414.2019.8940080" target="_blank" >http://dx.doi.org/10.1109/SENSORSNANO44414.2019.8940080</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/SENSORSNANO44414.2019.8940080" target="_blank" >10.1109/SENSORSNANO44414.2019.8940080</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements

Popis výsledku v původním jazyce

A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L-1) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.

Název v anglickém jazyce

Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements

Popis výsledku anglicky

A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L-1) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

2019 IEEE International Conference on Sensors and Nanotechnology

ISBN

978-1-5386-5619-8

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

1-4

Název nakladatele

American Institute of Physics and Magnetic Society of the IEEE

Místo vydání

San Francisco

Místo konání akce

Penang, Malaysia

Datum konání akce

24. 6. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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