HPLC-MS Methodology for R. carthamoides Extract Quality Evaluation: A Simultaneous Determination of Eight Bioactive Compounds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F22%3A00563744" target="_blank" >RIV/67985858:_____/22:00563744 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/22:43925288 RIV/60461373:22340/22:43925288

Výsledek na webu

<a href="https://www.mdpi.com/1424-2818/14/10/880" target="_blank" >https://www.mdpi.com/1424-2818/14/10/880</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/d14100880" target="_blank" >10.3390/d14100880</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HPLC-MS Methodology for R. carthamoides Extract Quality Evaluation: A Simultaneous Determination of Eight Bioactive Compounds

Popis výsledku v původním jazyce

Classified as an adaptogen, Maral root (Rhaponticum carthamoides, Leuzea carthamoides) is a herb that has long been used in Siberian as well as Russian alternative medicine. With over 200 substances found, this plant is a great source of bioactive compounds which have significant beneficial effects on human health and physical enhancement. Simultaneous quantification of the eight most therapeutic and abundant substances, i.e., 20-hydroxyecdysone (20-HE), kaempferol, hesperetin, quercetin, chlorogenic acid, N-feruloyl serotonin, cynaropicrin, and tracheloside belonging to various groups, such as ecdysteroids, flavonoids, phenolics, sesquiterpenes, and lignans, was performed for the first time through validated HPLC-MS. The evaluated parameters for method validation showed excellent linearity with R-2 higher than 0.996, stability under various environmental factors with % RSD <= 2%, and recovery between 97 and 103% for all the studied compounds. Other validation parameters including selectivity, sensitivity, and precision were found to be within the acceptance criteria. The results of the stability studies provide information on the best combination of conditions for sample handling and storage. Generally, for almost every compound, exposure to light and elevated temperature for 96 h led to degradation, nevertheless, the acidic environment was beneficial for most of them.

Název v anglickém jazyce

HPLC-MS Methodology for R. carthamoides Extract Quality Evaluation: A Simultaneous Determination of Eight Bioactive Compounds

Popis výsledku anglicky

Classified as an adaptogen, Maral root (Rhaponticum carthamoides, Leuzea carthamoides) is a herb that has long been used in Siberian as well as Russian alternative medicine. With over 200 substances found, this plant is a great source of bioactive compounds which have significant beneficial effects on human health and physical enhancement. Simultaneous quantification of the eight most therapeutic and abundant substances, i.e., 20-hydroxyecdysone (20-HE), kaempferol, hesperetin, quercetin, chlorogenic acid, N-feruloyl serotonin, cynaropicrin, and tracheloside belonging to various groups, such as ecdysteroids, flavonoids, phenolics, sesquiterpenes, and lignans, was performed for the first time through validated HPLC-MS. The evaluated parameters for method validation showed excellent linearity with R-2 higher than 0.996, stability under various environmental factors with % RSD <= 2%, and recovery between 97 and 103% for all the studied compounds. Other validation parameters including selectivity, sensitivity, and precision were found to be within the acceptance criteria. The results of the stability studies provide information on the best combination of conditions for sample handling and storage. Generally, for almost every compound, exposure to light and elevated temperature for 96 h led to degradation, nevertheless, the acidic environment was beneficial for most of them.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diversity

ISSN

1424-2818

e-ISSN

1424-2818

Svazek periodika

14

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

880

Kód UT WoS článku

000872644700001

EID výsledku v databázi Scopus

2-s2.0-85140823750