The strength of integrin binding between neutrophils and endothelial cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985874%3A_____%2F03%3A00375893" target="_blank" >RIV/67985874:_____/03:00375893 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s00249-003-0329-4" target="_blank" >http://dx.doi.org/10.1007/s00249-003-0329-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00249-003-0329-4" target="_blank" >10.1007/s00249-003-0329-4</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The strength of integrin binding between neutrophils and endothelial cells
Popis výsledku v původním jazyce
The firm adhesion of activated polymorphonuclear neutrophils to endothelial cells in blood vessels is achieved through binding of the integrin intercellular adhesion molecule. To contribute to the better understanding of this adhesion step, our investigation is aimed at the relationship between integrin expression and the strength of neutrophil binding to endothelial cells. Flow cytometry and 3D scanning microscopy are used to study integrin expression and distribution, respectively. It is found that CD11b/CD18 integrin expression is localized in clusters distributed irregularly over the neutrophil surface. After cell activation, the cluster distribution polarizes, increasing the local CD11b/CD18 density concurrently with nearly doubled integrin expression. The neutrophil adhesion efficiency is measured in a flow chamber coated successively by various substrates, including endothelial cells in an activated state. Analysis of the flow dependence of the number of attached cells reveals t
Název v anglickém jazyce
The strength of integrin binding between neutrophils and endothelial cells
Popis výsledku anglicky
The firm adhesion of activated polymorphonuclear neutrophils to endothelial cells in blood vessels is achieved through binding of the integrin intercellular adhesion molecule. To contribute to the better understanding of this adhesion step, our investigation is aimed at the relationship between integrin expression and the strength of neutrophil binding to endothelial cells. Flow cytometry and 3D scanning microscopy are used to study integrin expression and distribution, respectively. It is found that CD11b/CD18 integrin expression is localized in clusters distributed irregularly over the neutrophil surface. After cell activation, the cluster distribution polarizes, increasing the local CD11b/CD18 density concurrently with nearly doubled integrin expression. The neutrophil adhesion efficiency is measured in a flow chamber coated successively by various substrates, including endothelial cells in an activated state. Analysis of the flow dependence of the number of attached cells reveals t
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
BO - Biofyzika
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA305%2F01%2F1605" target="_blank" >GA305/01/1605: Vliv objevených shluků receptorů na regulaci adheze a transmigrace bílých krvinek</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2003
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
European Biophysics Journal With Biophysics Letters
ISSN
0175-7571
e-ISSN
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Svazek periodika
32
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
5
Strana od-do
684-688
Kód UT WoS článku
000187309600004
EID výsledku v databázi Scopus
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