Hsp70 Trap Assay for Detection of Misfolded Subproteome Related to Myelodysplastic Syndromes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F19%3A00518388" target="_blank" >RIV/67985882:_____/19:00518388 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00023736:_____/19:00012825
Výsledek na webu
<a href="https://pubs.acs.org/doi/10.1021/acs.analchem.9b04175" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.analchem.9b04175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.9b04175" target="_blank" >10.1021/acs.analchem.9b04175</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Hsp70 Trap Assay for Detection of Misfolded Subproteome Related to Myelodysplastic Syndromes
Popis výsledku v původním jazyce
The onset and progression of numerous serious diseases (e.g., various types of malignancies, neuro-degenerative diseases, and cardiac diseases) are, on a molecular level, associated with protein modifications and misfolding. Current methods for the detection of misfolded proteins are not able to detect the whole misfolded subproteome and, moreover, are rather laborious and time consuming. Herein, we report on a novel simple method for the detection of misfolded proteins employing a surface plasmon resonance (SPR) biosensor and heat shock protein 70 (Hsp70) that recognizes and traps misfolded proteins in a nucleotide-dependent manner. We use this method for the detection of misfolded proteins in blood plasma of patients with various subtypes of myelodysplastic syndromes (MDS) and healthy donors. Our results reveal significantly elevated levels of misfolded proteins in the two stages of MDS that are most affected by oxidative stress: low-risk (RARS) and intermediate-risk (RCMD) patients. This approach can be extended to a variety of diseases and provides unique insights into the thus far unexplored area of blood proteome.
Název v anglickém jazyce
Hsp70 Trap Assay for Detection of Misfolded Subproteome Related to Myelodysplastic Syndromes
Popis výsledku anglicky
The onset and progression of numerous serious diseases (e.g., various types of malignancies, neuro-degenerative diseases, and cardiac diseases) are, on a molecular level, associated with protein modifications and misfolding. Current methods for the detection of misfolded proteins are not able to detect the whole misfolded subproteome and, moreover, are rather laborious and time consuming. Herein, we report on a novel simple method for the detection of misfolded proteins employing a surface plasmon resonance (SPR) biosensor and heat shock protein 70 (Hsp70) that recognizes and traps misfolded proteins in a nucleotide-dependent manner. We use this method for the detection of misfolded proteins in blood plasma of patients with various subtypes of myelodysplastic syndromes (MDS) and healthy donors. Our results reveal significantly elevated levels of misfolded proteins in the two stages of MDS that are most affected by oxidative stress: low-risk (RARS) and intermediate-risk (RCMD) patients. This approach can be extended to a variety of diseases and provides unique insights into the thus far unexplored area of blood proteome.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytical Chemistry
ISSN
0003-2700
e-ISSN
—
Svazek periodika
91
Číslo periodika v rámci svazku
22
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
5
Strana od-do
14226-14230
Kód UT WoS článku
000498280100012
EID výsledku v databázi Scopus
2-s2.0-85074876787