Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F20%3A00538002" target="_blank" >RIV/67985882:_____/20:00538002 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.jphotobiol.2020.111812" target="_blank" >https://doi.org/10.1016/j.jphotobiol.2020.111812</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jphotobiol.2020.111812" target="_blank" >10.1016/j.jphotobiol.2020.111812</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers

Popis výsledku v původním jazyce

One of the most important barriers to the detection of the biological autoluminescence (BAL) from biosystems using a non-invasive monitoring approach, in both the in vivo and the in vitro applications, is its very low signal intensity ( < 1000 photons/s/cm(2)). Experimental studies have revealed that the formation of electron excited species, as a result of reactions of biomolecules with reactive oxygen species (ROS), is the principal biochemical source of the BAL which occurs during the cell metabolism. Mitochondria, as the most important organelles involved in oxidative metabolism, are considered to be the main intracellular BAL source. Hence, in order to achieve the BAL enhancement via affecting the mitochondria, we prepared a novel mitochondrial-liposomal nanocarrier with two attractive features including the intra-liposomal gold nanoparticle synthesizing ability and the mitochondria penetration capability. The results indicate that these nanocarriers (with the average size of 131.1 +/- 20.1 nm) are not only able to synthesize the gold nanoparticles within them (with the average size of 15 nm) and penetrate into the U2OS cell mitochondria, but they are also able to amplify the BAL signals. Our results open new possibilities for the use of biological autoluminescence as a non-invasive and label-free monitoring method in nanomedicine and biotechnology

Název v anglickém jazyce

Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers

Popis výsledku anglicky

One of the most important barriers to the detection of the biological autoluminescence (BAL) from biosystems using a non-invasive monitoring approach, in both the in vivo and the in vitro applications, is its very low signal intensity ( < 1000 photons/s/cm(2)). Experimental studies have revealed that the formation of electron excited species, as a result of reactions of biomolecules with reactive oxygen species (ROS), is the principal biochemical source of the BAL which occurs during the cell metabolism. Mitochondria, as the most important organelles involved in oxidative metabolism, are considered to be the main intracellular BAL source. Hence, in order to achieve the BAL enhancement via affecting the mitochondria, we prepared a novel mitochondrial-liposomal nanocarrier with two attractive features including the intra-liposomal gold nanoparticle synthesizing ability and the mitochondria penetration capability. The results indicate that these nanocarriers (with the average size of 131.1 +/- 20.1 nm) are not only able to synthesize the gold nanoparticles within them (with the average size of 15 nm) and penetrate into the U2OS cell mitochondria, but they are also able to amplify the BAL signals. Our results open new possibilities for the use of biological autoluminescence as a non-invasive and label-free monitoring method in nanomedicine and biotechnology

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20201 - Electrical and electronic engineering

Projekt

<a href="/cs/project/GA18-23597S" target="_blank" >GA18-23597S: Vysokofrekvenční mikrozařízení pro ovládání proteinových nanomotorů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Photochemistry and Photobiology. B - Biology Section

ISSN

1011-1344

e-ISSN

—

Svazek periodika

204

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

111812

Kód UT WoS článku

000518871200029

EID výsledku v databázi Scopus

2-s2.0-85079147459