Biological autoluminescence enables effective monitoring of yeast cell electroporation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F24%3A00585597" target="_blank" >RIV/67985882:_____/24:00585597 - isvavai.cz</a>

Výsledek na webu

<a href="https://analyticalsciencejournals-onlinelibrary-wiley-com.ezproxy.techlib.cz/doi/10.1002/biot.202300475" target="_blank" >https://analyticalsciencejournals-onlinelibrary-wiley-com.ezproxy.techlib.cz/doi/10.1002/biot.202300475</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/biot.202300475" target="_blank" >10.1002/biot.202300475</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biological autoluminescence enables effective monitoring of yeast cell electroporation

Popis výsledku v původním jazyce

The application of pulsed electric fields (PEFs) is becoming a promising tool for application in biotechnology, and the food industry. However, real-time monitoring of the efficiency of PEF treatment conditions is challenging, especially at the industrial scale and in continuous production conditions. To overcome this challenge, we have developed a straightforward setup capable of real-time detection of yeast biological autoluminescence (BAL) during pulsing. Saccharomyces cerevisiae culture was exposed to 8 pulses of 100 mu s width with electric field strength magnitude 2-7 kV cm-1. To assess the sensitivity of our method in detecting yeast electroporation, we conducted a comparison with established methods including impedance measurements, propidium iodide uptake, cell growth assay, and fluorescence microscopy. Our results demonstrate that yeast electroporation can be instantaneously monitored during pulsing, making it highly suitable for industrial applications. Furthermore, the simplicity of our setup facilitates its integration into continuous liquid flow systems. Additionally, we have established quantitative indicators based on a thorough statistical analysis of the data that can be implemented through a dedicated machine interface, providing efficiency indicators for analysis.

Název v anglickém jazyce

Biological autoluminescence enables effective monitoring of yeast cell electroporation

Popis výsledku anglicky

The application of pulsed electric fields (PEFs) is becoming a promising tool for application in biotechnology, and the food industry. However, real-time monitoring of the efficiency of PEF treatment conditions is challenging, especially at the industrial scale and in continuous production conditions. To overcome this challenge, we have developed a straightforward setup capable of real-time detection of yeast biological autoluminescence (BAL) during pulsing. Saccharomyces cerevisiae culture was exposed to 8 pulses of 100 mu s width with electric field strength magnitude 2-7 kV cm-1. To assess the sensitivity of our method in detecting yeast electroporation, we conducted a comparison with established methods including impedance measurements, propidium iodide uptake, cell growth assay, and fluorescence microscopy. Our results demonstrate that yeast electroporation can be instantaneously monitored during pulsing, making it highly suitable for industrial applications. Furthermore, the simplicity of our setup facilitates its integration into continuous liquid flow systems. Additionally, we have established quantitative indicators based on a thorough statistical analysis of the data that can be implemented through a dedicated machine interface, providing efficiency indicators for analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GX20-06873X" target="_blank" >GX20-06873X: SubTHz chipová zařízení pro řízení proteinových nanopřístrojů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotechnology Journal

ISSN

1860-6768

e-ISSN

1860-7314

Svazek periodika

19

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

e2300475

Kód UT WoS článku

001206049200001

EID výsledku v databázi Scopus

2-s2.0-85191046037