Assessment of Yeast Cells Electroporation by Autoluminescence and Impedance Measurements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F23%3A00584361" target="_blank" >RIV/67985882:_____/23:00584361 - isvavai.cz</a>

Výsledek na webu

<a href="https://ieeexplore-ieee-org.ezproxy.techlib.cz/document/10164612" target="_blank" >https://ieeexplore-ieee-org.ezproxy.techlib.cz/document/10164612</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.23919/MEASUREMENT59122.2023.10164612" target="_blank" >10.23919/MEASUREMENT59122.2023.10164612</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of Yeast Cells Electroporation by Autoluminescence and Impedance Measurements

Popis výsledku v původním jazyce

The application of pulsed electric field (PEF) is nowadays becoming a very promising tool for application in medicine or the food industry. However, the mechanisms of PEF interaction with living matter are still not fully elucidated. The aim of the presented work is to assess the electroporation phenomena in yeast cells using autoluminescence and impedance measurements. The yeast cell culture (Saccharomyces cerevisiae) is exposed to 8 pulses of 100 μs width. The corresponding electric field strength magnitude range is 2-7 kV.cm-1. The frequency of the pulsing is 2 Hz, and autoluminescence is monitored during exposure. The results indicate detectable differences in autoluminescence dynamics when PEF is applied. Due to its non-invasive and label-free application, the autoluminescence could be used as a beneficial tool for monitoring PEF biological effects

Název v anglickém jazyce

Assessment of Yeast Cells Electroporation by Autoluminescence and Impedance Measurements

Popis výsledku anglicky

The application of pulsed electric field (PEF) is nowadays becoming a very promising tool for application in medicine or the food industry. However, the mechanisms of PEF interaction with living matter are still not fully elucidated. The aim of the presented work is to assess the electroporation phenomena in yeast cells using autoluminescence and impedance measurements. The yeast cell culture (Saccharomyces cerevisiae) is exposed to 8 pulses of 100 μs width. The corresponding electric field strength magnitude range is 2-7 kV.cm-1. The frequency of the pulsing is 2 Hz, and autoluminescence is monitored during exposure. The results indicate detectable differences in autoluminescence dynamics when PEF is applied. Due to its non-invasive and label-free application, the autoluminescence could be used as a beneficial tool for monitoring PEF biological effects

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20201 - Electrical and electronic engineering

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 14 th International Conference on Measurement(2023)

ISBN

978-809726297-6

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

109

Název nakladatele

IEEE

Místo vydání

Bratislava

Místo konání akce

Smolenice

Datum konání akce

29. 5. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—