Interferometric scattering microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F25%3A00638159" target="_blank" >RIV/67985882:_____/25:00638159 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s43586-025-00391-1" target="_blank" >https://www.nature.com/articles/s43586-025-00391-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s43586-025-00391-1" target="_blank" >10.1038/s43586-025-00391-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interferometric scattering microscopy

Popis výsledku v původním jazyce

Over the past two decades, interferometric scattering (iSCAT) microscopy has become a powerful label-free imaging method with a range of applications in fundamental science and technology. iSCAT detects the scattering of subwavelength entities through interference with a reference beam of light. Performed in a variety of illumination and detection schemes, iSCAT has exploited both amplitude and phase information to reach single-molecule detection sensitivity, to determine the size, mass and refractive index of nanoparticles, to achieve high spatiotemporal precision in 3D tracking of nanoparticles, to image subcellular nanostructures, and to quantify ultrafast diffusion and transport of energy in solids. In this Primer, we describe the basic principles of iSCAT detection and imaging from theoretical and practical points of view. We discuss various factors that affect the attainable signal-to-noise ratio, which in turn determines crucial performance features such as sensitivity and speed. We survey selected applications in which iSCAT has been instrumental in providing new insights. Finally, we discuss some of the current challenges and potential avenues for advancing the technique further

Název v anglickém jazyce

Interferometric scattering microscopy

Popis výsledku anglicky

Over the past two decades, interferometric scattering (iSCAT) microscopy has become a powerful label-free imaging method with a range of applications in fundamental science and technology. iSCAT detects the scattering of subwavelength entities through interference with a reference beam of light. Performed in a variety of illumination and detection schemes, iSCAT has exploited both amplitude and phase information to reach single-molecule detection sensitivity, to determine the size, mass and refractive index of nanoparticles, to achieve high spatiotemporal precision in 3D tracking of nanoparticles, to image subcellular nanostructures, and to quantify ultrafast diffusion and transport of energy in solids. In this Primer, we describe the basic principles of iSCAT detection and imaging from theoretical and practical points of view. We discuss various factors that affect the attainable signal-to-noise ratio, which in turn determines crucial performance features such as sensitivity and speed. We survey selected applications in which iSCAT has been instrumental in providing new insights. Finally, we discuss some of the current challenges and potential avenues for advancing the technique further

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Reviews Methods Primers

ISSN

2662-8449

e-ISSN

2662-8449

Svazek periodika

5

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

21

Strana od-do

23

Kód UT WoS článku

001465461000002

EID výsledku v databázi Scopus

2-s2.0-105003428056