Three-dimensional interferometric scattering microscopy via remote focusing technique

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F20%3A00341425" target="_blank" >RIV/68407700:21460/20:00341425 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1364/OL.386172" target="_blank" >https://doi.org/10.1364/OL.386172</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1364/OL.386172" target="_blank" >10.1364/OL.386172</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Three-dimensional interferometric scattering microscopy via remote focusing technique

Popis výsledku v původním jazyce

Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visu- alize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We antici- pate that RF-iSCAT would enhance the utility of iSCAT for dynamics study.

Název v anglickém jazyce

Three-dimensional interferometric scattering microscopy via remote focusing technique

Popis výsledku anglicky

Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visu- alize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We antici- pate that RF-iSCAT would enhance the utility of iSCAT for dynamics study.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Optics Letters

ISSN

0146-9592

e-ISSN

1539-4794

Svazek periodika

45

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

2628-2631

Kód UT WoS článku

000535919200041

EID výsledku v databázi Scopus

2-s2.0-85084501291