Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F25%3A00638970" target="_blank" >RIV/67985882:_____/25:00638970 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/25:43927410 RIV/00216305:26620/26:0198636

Výsledek na webu

<a href="https://doi.org/10.1021/acsomega.5c05564" target="_blank" >https://doi.org/10.1021/acsomega.5c05564</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsomega.5c05564" target="_blank" >10.1021/acsomega.5c05564</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker

Popis výsledku v původním jazyce

The platinum nanozymes capped with tyrosine (Pt-Tyr NZ) were synthesized which demonstrated peroxidase and oxidase catalytic activities toward 3,3 ',5,5 '-tetramethylbenzidine (TMB). The kinetic analyses revealed that Pt-Tyr NZ exhibited higher catalytic activity and substrate affinity for both H2O2 and TMB compared to horseradish peroxidase (HRP), indicating its superior efficiency in biosensing applications. The synthesized Pt-Tyr NZ was reversibly conjugated with complementary DNA (cDNA), which can selectively hybridize with miRNA-21, a cancer biomarker, and after hybridization with miRNA-21, detaches from the surface of Pt-Tyr NZ. Upon cDNA conjugation, the catalytic activity of Pt-Tyr NZ decreased, but it was restored in the presence of miRNA-21, providing a one-step spectrophotometric biosensing method for miRNA-21 determination. Optimization of parameters affecting the sensitivity of the developed method for miRNA-21 detection was performed, including the tyrosine-to-platinum ratio in Pt-Tyr NZ synthesis, Pt-Tyr NZ concentration, and cDNA concentration for conjugation. The developed method was fully validated and showed promising results in terms of reproducibility (RSD = 1.26%, 95% confidence interval = 0.03, n = 6), repeatability (RSD = 0.83%, n = 5), and accuracy for spiked miRNA-21 determination in 10% filtered human serum samples (recovery rate = 109.7 +/- 4.2%). It showed a linear dynamic range (LDR) from 37.1 to 185 nM, with a limit of detection (LOD) of 11.1 nM and a limit of quantification (LOQ) of 36.9 nM

Název v anglickém jazyce

Tyrosine-Capped Pt Nanozyme Functionalized with cDNA: An Innovative Sensor Designed for One-Step Detection of the miRNA-21 Biomarker

Popis výsledku anglicky

The platinum nanozymes capped with tyrosine (Pt-Tyr NZ) were synthesized which demonstrated peroxidase and oxidase catalytic activities toward 3,3 ',5,5 '-tetramethylbenzidine (TMB). The kinetic analyses revealed that Pt-Tyr NZ exhibited higher catalytic activity and substrate affinity for both H2O2 and TMB compared to horseradish peroxidase (HRP), indicating its superior efficiency in biosensing applications. The synthesized Pt-Tyr NZ was reversibly conjugated with complementary DNA (cDNA), which can selectively hybridize with miRNA-21, a cancer biomarker, and after hybridization with miRNA-21, detaches from the surface of Pt-Tyr NZ. Upon cDNA conjugation, the catalytic activity of Pt-Tyr NZ decreased, but it was restored in the presence of miRNA-21, providing a one-step spectrophotometric biosensing method for miRNA-21 determination. Optimization of parameters affecting the sensitivity of the developed method for miRNA-21 detection was performed, including the tyrosine-to-platinum ratio in Pt-Tyr NZ synthesis, Pt-Tyr NZ concentration, and cDNA concentration for conjugation. The developed method was fully validated and showed promising results in terms of reproducibility (RSD = 1.26%, 95% confidence interval = 0.03, n = 6), repeatability (RSD = 0.83%, n = 5), and accuracy for spiked miRNA-21 determination in 10% filtered human serum samples (recovery rate = 109.7 +/- 4.2%). It showed a linear dynamic range (LDR) from 37.1 to 185 nM, with a limit of detection (LOD) of 11.1 nM and a limit of quantification (LOQ) of 36.9 nM

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Omega

ISSN

2470-1343

e-ISSN

2470-1343

Svazek periodika

10

Číslo periodika v rámci svazku

35

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

40324-40334

Kód UT WoS článku

001555731500001

EID výsledku v databázi Scopus

2-s2.0-105015513190