The comparison of eight different common in vitro and ex vivo environmentsnwith in vivo conditions applying model collagen samples: Correlationnpossibilities and their limits

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F24%3A00600196" target="_blank" >RIV/67985891:_____/24:00600196 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.polymertesting.2024.108621" target="_blank" >https://doi.org/10.1016/j.polymertesting.2024.108621</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.polymertesting.2024.108621" target="_blank" >10.1016/j.polymertesting.2024.108621</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The comparison of eight different common in vitro and ex vivo environmentsnwith in vivo conditions applying model collagen samples: Correlationnpossibilities and their limits

Popis výsledku v původním jazyce

New biomaterials are routinely evaluated for their degradation behaviour in the real body environment. Following the 3R strategy, in vitro simulated body conditions are often preferred. No studies that simultaneously compare such conditions with the real body environment have been conducted to date. Model porous collagen scaffolds were exposed for 21 days to eight different environments: simple salt-based and enzymatic media, human blood plasma, cell culture media with and without human fibroblasts and ex vivo model cortical bone, and subsequently compared with an in vivo environment represented by a pig peritoneum. The mechanical properties of the scaffolds were then determined via uniaxial compression testing, and the structural properties via the micro-CT, weight loss, infrared spectroscopy, X-ray diffraction and histological methods. Interestingly, the various analysed simulated body conditions caused differing alterations in the collagen scaffold characteristics when compared with the real body environment. The mechanical properties were similar during the first 7 days of incubation but diverged after 14 and 21 days. The structural properties varied significantly after just 7 days of incubation. The histological evaluation of the scaffolds exposed to the cellular, ex vivo and in vivo conditions revealed the poor ability of cells to completely populate the scaffolds, accompanied by the massive ingrowth of connective tissue into the in vivonexposed scaffolds, which resulted in their variable global behaviour. In conclusion, the value of in vitro simulated body environments lies in their screening capacity and feasibility, however, direct extrapolation to real body conditions needs to be verified going forward.

Název v anglickém jazyce

The comparison of eight different common in vitro and ex vivo environmentsnwith in vivo conditions applying model collagen samples: Correlationnpossibilities and their limits

Popis výsledku anglicky

New biomaterials are routinely evaluated for their degradation behaviour in the real body environment. Following the 3R strategy, in vitro simulated body conditions are often preferred. No studies that simultaneously compare such conditions with the real body environment have been conducted to date. Model porous collagen scaffolds were exposed for 21 days to eight different environments: simple salt-based and enzymatic media, human blood plasma, cell culture media with and without human fibroblasts and ex vivo model cortical bone, and subsequently compared with an in vivo environment represented by a pig peritoneum. The mechanical properties of the scaffolds were then determined via uniaxial compression testing, and the structural properties via the micro-CT, weight loss, infrared spectroscopy, X-ray diffraction and histological methods. Interestingly, the various analysed simulated body conditions caused differing alterations in the collagen scaffold characteristics when compared with the real body environment. The mechanical properties were similar during the first 7 days of incubation but diverged after 14 and 21 days. The structural properties varied significantly after just 7 days of incubation. The histological evaluation of the scaffolds exposed to the cellular, ex vivo and in vivo conditions revealed the poor ability of cells to completely populate the scaffolds, accompanied by the massive ingrowth of connective tissue into the in vivonexposed scaffolds, which resulted in their variable global behaviour. In conclusion, the value of in vitro simulated body environments lies in their screening capacity and feasibility, however, direct extrapolation to real body conditions needs to be verified going forward.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20601 - Medical engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Polymer Testing

ISSN

0142-9418

e-ISSN

1873-2348

Svazek periodika

140

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

17

Strana od-do

108621

Kód UT WoS článku

001348383400001

EID výsledku v databázi Scopus

2-s2.0-85207337208