Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10371399" target="_blank" >RIV/00216208:11110/18:10371399 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985891:_____/18:00497265 RIV/68407700:21220/18:00318334 RIV/00216208:11140/18:10371399 RIV/00064165:_____/18:10371399

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs10856-017-6024-2" target="_blank" >https://link.springer.com/article/10.1007%2Fs10856-017-6024-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10856-017-6024-2" target="_blank" >10.1007/s10856-017-6024-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?

Popis výsledku v původním jazyce

Collagen composite scaffolds have been used for a number of studies in tissue engineering. The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling. The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo. Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry. Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability. Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds. The elastic modulus and compressive strength of all the scaffolds decreased by ~95%. The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.

Název v anglickém jazyce

Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?

Popis výsledku anglicky

Collagen composite scaffolds have been used for a number of studies in tissue engineering. The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling. The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo. Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry. Hydration, simulating the conditions of cell seeding and cultivation up to 48 h and 576 h, was found to exert a minor effect on the morphological parameters and permeability. Conversely, hydration had a major statistically significant effect on the mechanical behaviour of all the tested scaffolds. The elastic modulus and compressive strength of all the scaffolds decreased by ~95%. The quantitative results provided confirm the importance of analysing scaffolds in the hydrated rather than the dry state since the former more precisely simulates the real environment for which such materials are designed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30306 - Sport and fitness sciences

Projekt

<a href="/cs/project/NV15-25813A" target="_blank" >NV15-25813A: Vývoj a komplexní preklinické testování nových kompozitních materiálů pro kostní chirurgii</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Materials Science: Materials in Medicine

ISSN

0957-4530

e-ISSN

—

Svazek periodika

29

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

000426609600002

EID výsledku v databázi Scopus

2-s2.0-85041447692