Plasmids of Selenomonas ruminantium and development of host-vector system

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F01%3A00347927" target="_blank" >RIV/67985904:_____/01:00347927 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/01:00348009

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plasmids of Selenomonas ruminantium and development of host-vector system

Popis výsledku v původním jazyce

A high frequency of plasmids was detected in the rumen bacterium Selenomonas ruminantium. Plasmids 0.9-20 kb in size were detected in more than 50 % tested strains. Densitometric analysis indicated that plasmid DNA could represents more than 25 % of total cellular DNA. Up to six plasmids were detected in strain S. ruminantium 18. Two smallest cryptic plasmids pSRD181 and pSRD182 from this strain were cloned into Escherichia coh vector pBluescriptSK(+) and partially characterized. The plasmid pSRD181 is1.4 kb and pSRD182 is 2.0 kb. While computer analysis of pSRD181 sequence data showed high homology with replication protein of Staphylococcus aureus plasmids, the pSRD182 sequence showed no significant homology in GenBank data, Strain S. ruminantium 28was successfully transformed with pJW1 derived plasmid pJIB1 using ampicillin resistance gene as marker This is the first report on transformation of selenomonads with foreign DNA.

Název v anglickém jazyce

Plasmids of Selenomonas ruminantium and development of host-vector system

Popis výsledku anglicky

A high frequency of plasmids was detected in the rumen bacterium Selenomonas ruminantium. Plasmids 0.9-20 kb in size were detected in more than 50 % tested strains. Densitometric analysis indicated that plasmid DNA could represents more than 25 % of total cellular DNA. Up to six plasmids were detected in strain S. ruminantium 18. Two smallest cryptic plasmids pSRD181 and pSRD182 from this strain were cloned into Escherichia coh vector pBluescriptSK(+) and partially characterized. The plasmid pSRD181 is1.4 kb and pSRD182 is 2.0 kb. While computer analysis of pSRD181 sequence data showed high homology with replication protein of Staphylococcus aureus plasmids, the pSRD182 sequence showed no significant homology in GenBank data, Strain S. ruminantium 28was successfully transformed with pJW1 derived plasmid pJIB1 using ampicillin resistance gene as marker This is the first report on transformation of selenomonads with foreign DNA.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

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Kód UT WoS článku

000171251900003

EID výsledku v databázi Scopus

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