PKB/AKT se podílí na znovuzahájení meiózy oocytů myši

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F06%3A00038826" target="_blank" >RIV/67985904:_____/06:00038826 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PKB/AKT is involved in resumption of meiosis in mouse oocytes

Popis výsledku v původním jazyce

Activation of PKB (protein kinase B) and CDK1 (cyclin-dependent kinase 1) during maturation of mouse oocytes was analysed. PKB phosphorylation and activation preceded GVBD (germinal vesicle breakdown) in oocytes maturing either in vitro or in vivo. Activation was transient and PKB activity was markedly reduced when virtually all of the oocytes had undergone GVBD. Treatment of oocytes with butyrolactone I, a specific inhibitor of CDK1, revealed that PKB activation is independent of CDK1 activity. LY-294002, an inhibitor of phosphoinositide 3-kinase?PKB signalling, suppressed activation of PKB and CDK1 as well as resumption of meiosis. During resumption of meiosis, PKB phosphorylated on Ser473 is associated with nuclear membrane and centrosome, whereas PKB phosphorylated on Thr308 is localized on centrosome only. The presence of phosphorylated PKB on centrosome at the time of GVBD suggests its important role for an initial CDK1 activation.

Název v anglickém jazyce

PKB/AKT is involved in resumption of meiosis in mouse oocytes

Popis výsledku anglicky

Activation of PKB (protein kinase B) and CDK1 (cyclin-dependent kinase 1) during maturation of mouse oocytes was analysed. PKB phosphorylation and activation preceded GVBD (germinal vesicle breakdown) in oocytes maturing either in vitro or in vivo. Activation was transient and PKB activity was markedly reduced when virtually all of the oocytes had undergone GVBD. Treatment of oocytes with butyrolactone I, a specific inhibitor of CDK1, revealed that PKB activation is independent of CDK1 activity. LY-294002, an inhibitor of phosphoinositide 3-kinase?PKB signalling, suppressed activation of PKB and CDK1 as well as resumption of meiosis. During resumption of meiosis, PKB phosphorylated on Ser473 is associated with nuclear membrane and centrosome, whereas PKB phosphorylated on Thr308 is localized on centrosome only. The presence of phosphorylated PKB on centrosome at the time of GVBD suggests its important role for an initial CDK1 activation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F03%2F0857" target="_blank" >GA523/03/0857: Úloha Akt kinázy pro regulaci G2/M fáze buněčného cyklu a získání vývojové kompetence oocytů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of the Cell

ISSN

0248-4900

e-ISSN

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Svazek periodika

98

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

13

Strana od-do

111-123

Kód UT WoS článku

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EID výsledku v databázi Scopus

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