Pro kondenzaci chromosomů během meotického zrání prasečích oocytů není nezbytná ani aktivita Aurory B ani fosforylace histonu H3

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F06%3A00040831" target="_blank" >RIV/67985904:_____/06:00040831 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes

Popis výsledku v původním jazyce

Aurora-B kinase is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora B kinase during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 mM Butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10mg/ml), inhibitor of proteosynthesis, results in a block of oocytes in t

Název v anglickém jazyce

Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes

Popis výsledku anglicky

Aurora-B kinase is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora B kinase during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 mM Butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10mg/ml), inhibitor of proteosynthesis, results in a block of oocytes in t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F03%2F0816" target="_blank" >GA204/03/0816: Charakterizace faktorů podílejích se na kondenzaci chromatinu během meiotického zrání prasečích a bovinních oocytů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of Reproduction

ISSN

0006-3363

e-ISSN

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Svazek periodika

74

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

905-912

Kód UT WoS článku

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EID výsledku v databázi Scopus

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