Diferenciace anaerobních polycentrických hub na základe rDNA PCR-RFLP analýzy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F06%3A00040840" target="_blank" >RIV/67985904:_____/06:00040840 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP

Popis výsledku v původním jazyce

The suitability of restriction fragment lenght polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orcinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments - nuclear small subunit 18S and the nuclear large subunit S28 and internal transcribed spacer (ITS)-were restricted with endonucleases AluI, HinfI, DraI. Although the SSU DNA fragment could be restricted successfully by all four enzymes, no differences were observed between restriction patterns of Orpinomyces and Anaeromyces. Genus-specific RFLP patterns were determined for Orpinomyces and Anaeromyces genera; the results showed that the PCR-RFLP analysis of rDNA offers an easy and rapid tool for differentiationof two polycentric genera of anaerobic fungi, which could be hardly separated on the basis of morphology.

Název v anglickém jazyce

Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP

Popis výsledku anglicky

The suitability of restriction fragment lenght polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orcinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments - nuclear small subunit 18S and the nuclear large subunit S28 and internal transcribed spacer (ITS)-were restricted with endonucleases AluI, HinfI, DraI. Although the SSU DNA fragment could be restricted successfully by all four enzymes, no differences were observed between restriction patterns of Orpinomyces and Anaeromyces. Genus-specific RFLP patterns were determined for Orpinomyces and Anaeromyces genera; the results showed that the PCR-RFLP analysis of rDNA offers an easy and rapid tool for differentiationof two polycentric genera of anaerobic fungi, which could be hardly separated on the basis of morphology.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F05%2F2555" target="_blank" >GA523/05/2555: Hloubková fylogeneze anaerobních hub</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

273-277

Kód UT WoS článku

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EID výsledku v databázi Scopus

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