Syntéza TNFAIP6 v předovulačních folikulech prasat. Sledování vlivu protilátky A38

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F08%3A00306895" target="_blank" >RIV/67985904:_____/08:00306895 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Synthesis of Tumor Necrosis Factor Alpha Induced Protein 6 in Porcine Preovulatory Follicles; A Study with A38 Antibody

Popis výsledku v původním jazyce

Our results, together with the high sequence similarity found among human, rabbit, mouse, rat, bovine and equine TNFAIP6 and the expression of this protein and/or the respective gene in preovulatory follicles of all the examined species, strongly supportthe concept that TNFAIP6-mediated HC covalent transfer onto hyaluronic acid (HA) synthesized by cumulus cells is a mechanism common to mammalian OCCs. However, in contrast to the situation observed in the mouse, anti-TNFAIP6 A38 antibody did not preventcumulus expansion nor HA accumulation in the matrix of pig OCCs stimulated with FSH. It suggests that porcine OCCs have evolved supplemental mechanisms for holding HA in the matrix. Thus, it is reasonable to hypothesize that different relative levels ofHA, TNFAIP6, versican as well as other matrix components are synthesized by cumulus cells of different species, with the aim to create for each species the matrix that is the most suitable for ovulation and oocyte fertilization.

Název v anglickém jazyce

Synthesis of Tumor Necrosis Factor Alpha Induced Protein 6 in Porcine Preovulatory Follicles; A Study with A38 Antibody

Popis výsledku anglicky

Our results, together with the high sequence similarity found among human, rabbit, mouse, rat, bovine and equine TNFAIP6 and the expression of this protein and/or the respective gene in preovulatory follicles of all the examined species, strongly supportthe concept that TNFAIP6-mediated HC covalent transfer onto hyaluronic acid (HA) synthesized by cumulus cells is a mechanism common to mammalian OCCs. However, in contrast to the situation observed in the mouse, anti-TNFAIP6 A38 antibody did not preventcumulus expansion nor HA accumulation in the matrix of pig OCCs stimulated with FSH. It suggests that porcine OCCs have evolved supplemental mechanisms for holding HA in the matrix. Thus, it is reasonable to hypothesize that different relative levels ofHA, TNFAIP6, versican as well as other matrix components are synthesized by cumulus cells of different species, with the aim to create for each species the matrix that is the most suitable for ovulation and oocyte fertilization.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA305%2F05%2F0960" target="_blank" >GA305/05/0960: Imunodetekce strukturalních komponent kumulu-oophoru, nezbytných pro úspěšné oplození.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of Reproduction

ISSN

0006-3363

e-ISSN

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Svazek periodika

78

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

903-909

Kód UT WoS článku

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EID výsledku v databázi Scopus

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