Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F09%3A00332768" target="_blank" >RIV/67985904:_____/09:00332768 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

Popis výsledku v původním jazyce

Tumor necrosis factor alpha -induced protein 6 (TNFAIP6) is essential for formation of the cumulus extracellular matrix and female fertility. We studied its expression in porcine preovulatory follicles. The levels of TNFAIP6 mRNA were low in follicles onday 12 and day 15 of the estrous cycle and at 66 h after eCG stimulation but were significantly increased at 4 h after hCG. The high level of TNFAIP6 expression was maintained until 16 h after hCG stimulation and gradually decreased at 24 and 32 h afterhCG. During in vitro culture, FSH/LH-induced TNFAIP6 mRNA was expressed in both OCCs and MGCs in a similar temporal pattern as seen in vivo. We conclude that TNFAIP6 expression in the pig, like other species, increases in preovulatory follicles following the LH(hCG) surge. The OCC and MGC display similar patterns of TNFAIP6 expression under both in vivo and in vitro conditions.

Název v anglickém jazyce

Expression of Tumor Necrosis Factor Alpha-Included Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

Popis výsledku anglicky

Tumor necrosis factor alpha -induced protein 6 (TNFAIP6) is essential for formation of the cumulus extracellular matrix and female fertility. We studied its expression in porcine preovulatory follicles. The levels of TNFAIP6 mRNA were low in follicles onday 12 and day 15 of the estrous cycle and at 66 h after eCG stimulation but were significantly increased at 4 h after hCG. The high level of TNFAIP6 expression was maintained until 16 h after hCG stimulation and gradually decreased at 24 and 32 h afterhCG. During in vitro culture, FSH/LH-induced TNFAIP6 mRNA was expressed in both OCCs and MGCs in a similar temporal pattern as seen in vivo. We conclude that TNFAIP6 expression in the pig, like other species, increases in preovulatory follicles following the LH(hCG) surge. The OCC and MGC display similar patterns of TNFAIP6 expression under both in vivo and in vitro conditions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F08%2F0111" target="_blank" >GA523/08/0111: Potenciál peptidů podobných růstovému epidermálnímu faktoru a analogů cAMP zlepšit vývojovou kapacitu oocytů prasete po kultivaci in vitro</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Reproduction and Development

ISSN

0916-8818

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

00026738780000

EID výsledku v databázi Scopus

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