Identifikace odlišně exprimovaných genů při spontánní regresi melanomu u prasečího modelu MeLiM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F08%3A00308688" target="_blank" >RIV/67985904:_____/08:00308688 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of differentially expressed genes in spontaneously regressing melanoma using the MeLiM Swine Model

Popis výsledku v původním jazyce

Partial and rarely complete spontaneous regression have been observed in cutaneous melanoma patients but little is known about the molecular mechanisms involved. The Melanoblastoma-bearing Libechov Minipig (MeLiM) is a suitable model to study the spontaneous regression because MeLiM pigs exhibit naturally occurring melanomas which regress completely 6 months after birth. In this study, we used suppression subtractive hybridization (SSH) to identify molecular determinants of melanoma regression within swine melanoma tissues and melanoma cell cultures. Several markers involved in cell-adhesion,-communication,-motility, signal transduction, negative regulation of cell proliferation and immune response were identified that correlated with melanoma regression. We identified the retinoic acid responder 1 gene (RARRES1) as a main actor of the regression in melanoma. For the most differentially expressed genes, we validated the results obtained by SSH with qRT-PCR and immunohistochemistry.

Název v anglickém jazyce

Identification of differentially expressed genes in spontaneously regressing melanoma using the MeLiM Swine Model

Popis výsledku anglicky

Partial and rarely complete spontaneous regression have been observed in cutaneous melanoma patients but little is known about the molecular mechanisms involved. The Melanoblastoma-bearing Libechov Minipig (MeLiM) is a suitable model to study the spontaneous regression because MeLiM pigs exhibit naturally occurring melanomas which regress completely 6 months after birth. In this study, we used suppression subtractive hybridization (SSH) to identify molecular determinants of melanoma regression within swine melanoma tissues and melanoma cell cultures. Several markers involved in cell-adhesion,-communication,-motility, signal transduction, negative regulation of cell proliferation and immune response were identified that correlated with melanoma regression. We identified the retinoic acid responder 1 gene (RARRES1) as a main actor of the regression in melanoma. For the most differentially expressed genes, we validated the results obtained by SSH with qRT-PCR and immunohistochemistry.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pigment Cell and Melanoma Research

ISSN

1755-1471

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

15

Strana od-do

147-161

Kód UT WoS článku

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EID výsledku v databázi Scopus

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