Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F13%3A00397823" target="_blank" >RIV/67985904:_____/13:00397823 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fertnstert.2012.12.040" target="_blank" >http://dx.doi.org/10.1016/j.fertnstert.2012.12.040</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fertnstert.2012.12.040" target="_blank" >10.1016/j.fertnstert.2012.12.040</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium

Popis výsledku v původním jazyce

Objective: To determine whether inhibition of epidermal growth factor (EGF) receptor tyrosine kinase with lapatinib affects oocyte maturation, expression of the cumulus expansion-associated genes such as tumor necrosis factor alpha-induced protein 6 (TNFAIP6) and prostaglandin-endoperoxide synthase 2 (PTGS2), and synthesis of hyaluronan (HA) and progesterone (P) by porcine oocyte cumulus complexes (OCC). Design: Our work focuses on lapatinib, an orally active small molecule that selectively inhibits thetyrosine kinase domain of both EGF receptor and human EGF receptor 2, and downstream signaling. Setting: A reproductive biology laboratory. Patient(s): Not applicable. Intervention(s): Porcine OCC were cultured in vitro in a medium with FSH/LH in the presence/absence of lapatinib. Main Outcome Measure(s): Methods performed: real-time reverse transcriptase-polymerase chain reaction (PCR), immunofluorescence, RIA. Result(s): In FSH/LH-stimulated and expanded cumulus oophorus extracellular

Název v anglickém jazyce

Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium

Popis výsledku anglicky

Objective: To determine whether inhibition of epidermal growth factor (EGF) receptor tyrosine kinase with lapatinib affects oocyte maturation, expression of the cumulus expansion-associated genes such as tumor necrosis factor alpha-induced protein 6 (TNFAIP6) and prostaglandin-endoperoxide synthase 2 (PTGS2), and synthesis of hyaluronan (HA) and progesterone (P) by porcine oocyte cumulus complexes (OCC). Design: Our work focuses on lapatinib, an orally active small molecule that selectively inhibits thetyrosine kinase domain of both EGF receptor and human EGF receptor 2, and downstream signaling. Setting: A reproductive biology laboratory. Patient(s): Not applicable. Intervention(s): Porcine OCC were cultured in vitro in a medium with FSH/LH in the presence/absence of lapatinib. Main Outcome Measure(s): Methods performed: real-time reverse transcriptase-polymerase chain reaction (PCR), immunofluorescence, RIA. Result(s): In FSH/LH-stimulated and expanded cumulus oophorus extracellular

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP502%2F11%2F0593" target="_blank" >GAP502/11/0593: Signální dráhy a geny regulující gonadotropiny indukované zrání oocyt-kumulárních komplexů prasete</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fertility and Sterility

ISSN

0015-0282

e-ISSN

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Svazek periodika

99

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1739-1748

Kód UT WoS článku

000318612400044

EID výsledku v databázi Scopus

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