Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F14%3A00427357" target="_blank" >RIV/67985904:_____/14:00427357 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.domaniend.2014.01.003" target="_blank" >http://dx.doi.org/10.1016/j.domaniend.2014.01.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.domaniend.2014.01.003" target="_blank" >10.1016/j.domaniend.2014.01.003</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
Popis výsledku v původním jazyce
This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan(HA) and heavy chains of inter-? trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor ?-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were basedon freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100ng/mL) inthe presence/absence of RU486 or indomethacin. Western blot analysis, 3H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 ?M) caused a decrease in the number of oocyt
Název v anglickém jazyce
Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
Popis výsledku anglicky
This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan(HA) and heavy chains of inter-? trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor ?-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were basedon freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100ng/mL) inthe presence/absence of RU486 or indomethacin. Western blot analysis, 3H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 ?M) caused a decrease in the number of oocyt
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
ED - Fyziologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/GAP502%2F11%2F0593" target="_blank" >GAP502/11/0593: Signální dráhy a geny regulující gonadotropiny indukované zrání oocyt-kumulárních komplexů prasete</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Domestic Animal Endocrinology
ISSN
0739-7240
e-ISSN
—
Svazek periodika
48
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
7-14
Kód UT WoS článku
000337643900002
EID výsledku v databázi Scopus
—