Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F15%3A00440351" target="_blank" >RIV/67985904:_____/15:00440351 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/15:#0001287 RIV/68378050:_____/15:00440351

Výsledek na webu

<a href="http://dx.doi.org/10.1038/ncomms7078" target="_blank" >http://dx.doi.org/10.1038/ncomms7078</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/ncomms7078" target="_blank" >10.1038/ncomms7078</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway

Popis výsledku v původním jazyce

The fully-grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during early development. However, we find that an abundant RNA population is retained in the oocyte nucleus and contains specific mRNAs important for meiotic progression. During the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region that previously surrounded the nucleus. These distinct translational hotspots are separated by endoplasmic reticulum and Lamin, and disappear following polar body extrusion. Chromosomal translational hotspots are controlled by the activity of the mTOR–eIF4F pathway. Here, we reveal a mechanism that—following the resumption of meiosis—controls the temporal and spatial translation of a specific set of transcripts required for normal spindle assembly, chromosome alignment and segregation.

Název v anglickém jazyce

Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway

Popis výsledku anglicky

The fully-grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during early development. However, we find that an abundant RNA population is retained in the oocyte nucleus and contains specific mRNAs important for meiotic progression. During the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region that previously surrounded the nucleus. These distinct translational hotspots are separated by endoplasmic reticulum and Lamin, and disappear following polar body extrusion. Chromosomal translational hotspots are controlled by the activity of the mTOR–eIF4F pathway. Here, we reveal a mechanism that—following the resumption of meiosis—controls the temporal and spatial translation of a specific set of transcripts required for normal spindle assembly, chromosome alignment and segregation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Communications

ISSN

2041-1723

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

6078

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000348831300005

EID výsledku v databázi Scopus

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