31P MR Spectroscopy of the Testes and Immunohistochemical Analysis of Sperm 
of Transgenic Boars Carried N terminal Part of Human Mutated Huntingtin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F15%3A00476983" target="_blank" >RIV/67985904:_____/15:00476983 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/15:00452777 RIV/00023001:_____/15:00059489

Výsledek na webu

<a href="http://dx.doi.org/10.14735/amcsnn20152S28" target="_blank" >http://dx.doi.org/10.14735/amcsnn20152S28</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14735/amcsnn20152S28" target="_blank" >10.14735/amcsnn20152S28</a>

Jazyk výsledku

angličtina

Název v původním jazyce

31P MR Spectroscopy of the Testes and Immunohistochemical Analysis of Sperm 
of Transgenic Boars Carried N terminal Part of Human Mutated Huntingtin

Popis výsledku v původním jazyce

Huntington’s disease (HD) is an inherited autosomal neurodegenerative disorder characterized by motor dysfunctions, behavioral and cognitive disturbances. It affects predominantly the brain, however, changes were found also in peripheral tissues. Some of these changes can result from direct expression of mutant huntingtin; its highest levels have been found in the brain and testes. In 2009 we established a minipig model of HD (TgHD) expressing N terminal (548aa) part of human mutated huntingtin encoded 124 CAG/ CAA repeats. Previous research has revealed the presence of reduced fertility and fewer spermatozoa per ejaculate in TgHD boars started at 13 months of age. The aim of this study was to determine changes in the testes of 24 months old transgenic boars (F2 generation in vivo) using non invasive methodology of 31P magnetic resonance (MR) spectroscopy as well as to perform imunohistochemical analysis of TgHD sperm collected fromform F1 and F3 generation before HD onset. The results have shown significant reduction of relative phosphodiester concentration in testicular parenchyma of TgHD boars compared to wild type (WT) ones of the same ages. Moreover immunohistochemical analysis of sperm collected from TgHD and WT have revealed exclusive anti polyQ specific (clone 3B5H10) as well as significantly increased anti huntingtin (clone EPR5526) staining in transgenic spermatozoa tails in comparison with WT counterparts. Thus, our results are suggestive of the negative impact of human mutated huntingtin on testes metabolism as well as sperm abnormalities.

Název v anglickém jazyce

31P MR Spectroscopy of the Testes and Immunohistochemical Analysis of Sperm 
of Transgenic Boars Carried N terminal Part of Human Mutated Huntingtin

Popis výsledku anglicky

Huntington’s disease (HD) is an inherited autosomal neurodegenerative disorder characterized by motor dysfunctions, behavioral and cognitive disturbances. It affects predominantly the brain, however, changes were found also in peripheral tissues. Some of these changes can result from direct expression of mutant huntingtin; its highest levels have been found in the brain and testes. In 2009 we established a minipig model of HD (TgHD) expressing N terminal (548aa) part of human mutated huntingtin encoded 124 CAG/ CAA repeats. Previous research has revealed the presence of reduced fertility and fewer spermatozoa per ejaculate in TgHD boars started at 13 months of age. The aim of this study was to determine changes in the testes of 24 months old transgenic boars (F2 generation in vivo) using non invasive methodology of 31P magnetic resonance (MR) spectroscopy as well as to perform imunohistochemical analysis of TgHD sperm collected fromform F1 and F3 generation before HD onset. The results have shown significant reduction of relative phosphodiester concentration in testicular parenchyma of TgHD boars compared to wild type (WT) ones of the same ages. Moreover immunohistochemical analysis of sperm collected from TgHD and WT have revealed exclusive anti polyQ specific (clone 3B5H10) as well as significantly increased anti huntingtin (clone EPR5526) staining in transgenic spermatozoa tails in comparison with WT counterparts. Thus, our results are suggestive of the negative impact of human mutated huntingtin on testes metabolism as well as sperm abnormalities.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Česká a Slovenská neurologie a neurochirurgie

ISSN

1210-7859

e-ISSN

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Svazek periodika

78

Číslo periodika v rámci svazku

Suppl 2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

28-33

Kód UT WoS článku

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EID výsledku v databázi Scopus

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