The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00525646" target="_blank" >RIV/67985904:_____/20:00525646 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/20:00525646

Výsledek na webu

<a href="https://academic.oup.com/nar/article/48/6/3211/5709715" target="_blank" >https://academic.oup.com/nar/article/48/6/3211/5709715</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkz1239" target="_blank" >10.1093/nar/gkz1239</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution

Popis výsledku v původním jazyce

Tens of thousands of rapidly evolving long non-coding RNA (lncRNA) genes have been identified, but functions were assigned to relatively few of them. The lncRNA contribution to the mouse oocyte physiology remains unknown. We report the evolutionary history and functional analysis of Sirena1, the most expressed lncRNA and the 10th most abundant poly(A) transcript in mouse oocytes. Sirena1 appeared in the common ancestor of mouse and rat and became engaged in two different post-transcriptional regulations. First, antisense oriented Bob pseudogene insertion into Sirena1 exon 1 is a source of small RNAs targeting Sob mRNA via RNA interference. Second, Sirena1 evolved functional cytoplasmic polyadenylation elements, an unexpected feature borrowed from translation control of specific maternal mRNAs. Sirena1 knock-out does not affect fertility, but causes minor dysregulation of the maternal transcriptome. This includes increased levels of Elob and mitochondrial mRNAs. Mitochondria in Sirena1(-/-) oocytes disperse from the perinuclear compartment, but do not change in number or ultrastructure. Taken together, Sirenal contributes to RNA interference and mitochondrial aggregation in mouse oocytes. Sirena1 exemplifies how lncRNAs stochastically engage or even repurpose molecular mechanisms during evolution. Simultaneously, Sirenai1 expression levels and unique functional features contrast with the lack of functional importance assessed under laboratory conditions.

Název v anglickém jazyce

The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution

Popis výsledku anglicky

Tens of thousands of rapidly evolving long non-coding RNA (lncRNA) genes have been identified, but functions were assigned to relatively few of them. The lncRNA contribution to the mouse oocyte physiology remains unknown. We report the evolutionary history and functional analysis of Sirena1, the most expressed lncRNA and the 10th most abundant poly(A) transcript in mouse oocytes. Sirena1 appeared in the common ancestor of mouse and rat and became engaged in two different post-transcriptional regulations. First, antisense oriented Bob pseudogene insertion into Sirena1 exon 1 is a source of small RNAs targeting Sob mRNA via RNA interference. Second, Sirena1 evolved functional cytoplasmic polyadenylation elements, an unexpected feature borrowed from translation control of specific maternal mRNAs. Sirena1 knock-out does not affect fertility, but causes minor dysregulation of the maternal transcriptome. This includes increased levels of Elob and mitochondrial mRNAs. Mitochondria in Sirena1(-/-) oocytes disperse from the perinuclear compartment, but do not change in number or ultrastructure. Taken together, Sirenal contributes to RNA interference and mitochondrial aggregation in mouse oocytes. Sirena1 exemplifies how lncRNAs stochastically engage or even repurpose molecular mechanisms during evolution. Simultaneously, Sirenai1 expression levels and unique functional features contrast with the lack of functional importance assessed under laboratory conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

3211-3227

Kód UT WoS článku

000525957500035

EID výsledku v databázi Scopus

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