Laser microirradiation as a tool for DNA damage response studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00536629" target="_blank" >RIV/67985904:_____/20:00536629 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Laser microirradiation as a tool for DNA damage response studies

Popis výsledku v původním jazyce

Double-strand DNA breaks (DSBs) are considered as the most hazardous type of DNA lesions. Recognition of DSBs, temporal cell cycle arrest and activation of repair pathways are crucial steps in response to DNA damage. Errors in any of these steps can lead to adverse consequences affecting genome integrity, e.g. chromosomal aberrations or generation of mutations. The genomic instability caused by severe endpoints of DSBs processing can lead to cell death or development of cancer. Therefore, it is crucial to study all aspects of DNA damage response in cells.nIn the context of studying DNA damage response in cells, DSBs are typically induced either by γ-irradiation or by UV light. DSBs can be also induced by radiomimetic drugs, such as Neocarzinostatin. However, these techniques have several limitations, e.g. inability to induced DSBs only in a specific region of nucleus. Therefore, the laser UV microirradiation technique combined with confocal microscopy was developed to study the DNA damage response in living cells.

Název v anglickém jazyce

Laser microirradiation as a tool for DNA damage response studies

Popis výsledku anglicky

Double-strand DNA breaks (DSBs) are considered as the most hazardous type of DNA lesions. Recognition of DSBs, temporal cell cycle arrest and activation of repair pathways are crucial steps in response to DNA damage. Errors in any of these steps can lead to adverse consequences affecting genome integrity, e.g. chromosomal aberrations or generation of mutations. The genomic instability caused by severe endpoints of DSBs processing can lead to cell death or development of cancer. Therefore, it is crucial to study all aspects of DNA damage response in cells.nIn the context of studying DNA damage response in cells, DSBs are typically induced either by γ-irradiation or by UV light. DSBs can be also induced by radiomimetic drugs, such as Neocarzinostatin. However, these techniques have several limitations, e.g. inability to induced DSBs only in a specific region of nucleus. Therefore, the laser UV microirradiation technique combined with confocal microscopy was developed to study the DNA damage response in living cells.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/LO1609" target="_blank" >LO1609: Modely závažných lidských onemocnění: Traumatické poškození míchy, Huntingtonova choroba, melanom a neplodnost</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Translational Research in Serious Human Diseases

ISBN

978-80-200-3158-7

Počet stran výsledku

8

Strana od-do

257-264

Počet stran knihy

271

Název nakladatele

Academia

Místo vydání

Praha

Kód UT WoS kapitoly

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