Microbial shifts of faecal microbiota using enteral nutrition in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00541223" target="_blank" >RIV/67985904:_____/21:00541223 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/21:10419481 RIV/00216208:11130/21:10419481 RIV/60460709:41210/21:84088

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1756464620305545" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1756464620305545</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jff.2020.104330" target="_blank" >10.1016/j.jff.2020.104330</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microbial shifts of faecal microbiota using enteral nutrition in vitro

Popis výsledku v původním jazyce

Enteral nutrition (EN) formulas of polymeric type ordinarily have similar content of intact macronutrients but may vary in prebiotic saccharides and micronutrients. These components can play an important role in the intestinal microbiota modulation. The aim of this study was to investigate microbial changes of faecal samples after their in vitro anaerobic cultivation in four polymeric EN formulas using plate technique method, metabolite analysis, and microbiota profiling using 16S rRNA sequencing. Detected cultivable commensal groups (bifidobacteria, lactobacilli, Escherichia coli) in faecal samples of donors were able to grow in EN formulas. However, their counts varied depending on the individual donor and the type of EN formula. Similar trend was found in detected metabolites such as acetate, lactate, and butyrate. Also, taxonomic composition and diversity of original and cultivated faecal microbiota of one individual on different EN formula indicate a possible effect of the prebiotics and micronutrients to modulate gut microbiota.

Název v anglickém jazyce

Microbial shifts of faecal microbiota using enteral nutrition in vitro

Popis výsledku anglicky

Enteral nutrition (EN) formulas of polymeric type ordinarily have similar content of intact macronutrients but may vary in prebiotic saccharides and micronutrients. These components can play an important role in the intestinal microbiota modulation. The aim of this study was to investigate microbial changes of faecal samples after their in vitro anaerobic cultivation in four polymeric EN formulas using plate technique method, metabolite analysis, and microbiota profiling using 16S rRNA sequencing. Detected cultivable commensal groups (bifidobacteria, lactobacilli, Escherichia coli) in faecal samples of donors were able to grow in EN formulas. However, their counts varied depending on the individual donor and the type of EN formula. Similar trend was found in detected metabolites such as acetate, lactate, and butyrate. Also, taxonomic composition and diversity of original and cultivated faecal microbiota of one individual on different EN formula indicate a possible effect of the prebiotics and micronutrients to modulate gut microbiota.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Functional Foods

ISSN

1756-4646

e-ISSN

2214-9414

Svazek periodika

77

Číslo periodika v rámci svazku

FEB 2021

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

104330

Kód UT WoS článku

000614472600001

EID výsledku v databázi Scopus

2-s2.0-85098588929