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Chronic Hepatitis C Virus Infection Modulates the Transcriptional Profiles of CD4(+) T Cells

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00541961" target="_blank" >RIV/67985904:_____/21:00541961 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/00216208:11150/21:10427910 RIV/61383082:_____/21:00001065 RIV/00216208:11110/21:10427910 RIV/00064211:_____/21:W0000024 RIV/00179906:_____/21:10427910

  • Výsledek na webu

    <a href="https://www.hindawi.com/journals/cjidmm/2021/6689834/" target="_blank" >https://www.hindawi.com/journals/cjidmm/2021/6689834/</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1155/2021/6689834" target="_blank" >10.1155/2021/6689834</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Chronic Hepatitis C Virus Infection Modulates the Transcriptional Profiles of CD4(+) T Cells

  • Popis výsledku v původním jazyce

    Background. Chronic hepatitis C (CHC) is associated with altered cell-mediated immune response. Objective. The aim of the study was to characterize functional alterations in CD4(+) T cell subsets and myeloid-derived suppressor cells (MDSCs) during chronic hepatitis C virus (HCV) infection. Methodology. The expression levels of the lineage-defining transcriptional factors (TFs) T-bet, Gata3, Roryt, and Foxp3 in circulating CD4(+) T cells and percentages of MDSCs in peripheral blood were evaluated in 33 patients with CHC, 31 persons, who had spontaneously cleared the HCV infection, and 30 healthy subjects. Analysis. The CD4(+) T cells TFs T-bet (T-box expressed in T cells), Foxp3 (Forkhead box P3 transcription factor), Gata3 (Gata-binding protein 3), and Roryt (retinoic-acid-related orphan receptor gamma) and activation of CD8(+) T cells, as well as percentages of MDSCs, were measured by multicolor flow cytometry after intracellular and surface staining of peripheral blood mononuclear cells with fluorescent monoclonal antibodies. Result. The patients with CHC had significantly lower percentages of CD4(+) T cells expressing Roryt and Gata3 and higher percentages of Foxp3-expressing CD4(+) T cells than healthy controls and persons who spontaneously cleared HCV infection. The ratios of T-bet(+)/Gata3(+) and Foxp3(+)/Ror gamma t(+) CD4(+) T cells were the highest in the patients with CHC. In the patients with CHC, the percentages of Gata3(+) and Ror gamma t CD4(+) T cells and the percentages of T-bet(+) CD4(+) T cells and CD38(+)/ HLA-DR+ CD8(+) T cells demonstrated significant positive correlations. In addition, the percentage of CD38(+)/HLA-DR+ CD8(+) T cells correlated negatively with the percentage of MDSCs. Conclusion. Chronic HCV infection is associated with downregulation of TFs Gata3 and Roryt polarizing CD4+ T cells into Th2 and Th17 phenotypes together with upregulation of Foxp3 responsible for induction of regulatory T cells suppressing immune response.

  • Název v anglickém jazyce

    Chronic Hepatitis C Virus Infection Modulates the Transcriptional Profiles of CD4(+) T Cells

  • Popis výsledku anglicky

    Background. Chronic hepatitis C (CHC) is associated with altered cell-mediated immune response. Objective. The aim of the study was to characterize functional alterations in CD4(+) T cell subsets and myeloid-derived suppressor cells (MDSCs) during chronic hepatitis C virus (HCV) infection. Methodology. The expression levels of the lineage-defining transcriptional factors (TFs) T-bet, Gata3, Roryt, and Foxp3 in circulating CD4(+) T cells and percentages of MDSCs in peripheral blood were evaluated in 33 patients with CHC, 31 persons, who had spontaneously cleared the HCV infection, and 30 healthy subjects. Analysis. The CD4(+) T cells TFs T-bet (T-box expressed in T cells), Foxp3 (Forkhead box P3 transcription factor), Gata3 (Gata-binding protein 3), and Roryt (retinoic-acid-related orphan receptor gamma) and activation of CD8(+) T cells, as well as percentages of MDSCs, were measured by multicolor flow cytometry after intracellular and surface staining of peripheral blood mononuclear cells with fluorescent monoclonal antibodies. Result. The patients with CHC had significantly lower percentages of CD4(+) T cells expressing Roryt and Gata3 and higher percentages of Foxp3-expressing CD4(+) T cells than healthy controls and persons who spontaneously cleared HCV infection. The ratios of T-bet(+)/Gata3(+) and Foxp3(+)/Ror gamma t(+) CD4(+) T cells were the highest in the patients with CHC. In the patients with CHC, the percentages of Gata3(+) and Ror gamma t CD4(+) T cells and the percentages of T-bet(+) CD4(+) T cells and CD38(+)/ HLA-DR+ CD8(+) T cells demonstrated significant positive correlations. In addition, the percentage of CD38(+)/HLA-DR+ CD8(+) T cells correlated negatively with the percentage of MDSCs. Conclusion. Chronic HCV infection is associated with downregulation of TFs Gata3 and Roryt polarizing CD4+ T cells into Th2 and Th17 phenotypes together with upregulation of Foxp3 responsible for induction of regulatory T cells suppressing immune response.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10603 - Genetics and heredity (medical genetics to be 3)

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/NT14072" target="_blank" >NT14072: Prediktivní imunologické markery u pacientů s infekcí virem hepatitidy C</a><br>

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Canadian Journal of Infectious Diseases & Medical Microbiology

  • ISSN

    1712-9532

  • e-ISSN

    1918-1493

  • Svazek periodika

    2021

  • Číslo periodika v rámci svazku

    MAR 12

  • Stát vydavatele periodika

    CA - Kanada

  • Počet stran výsledku

    6

  • Strana od-do

    6689834

  • Kód UT WoS článku

    000631966800001

  • EID výsledku v databázi Scopus

    2-s2.0-85102987482