Oocyte specific lncRNA variant Rose influences oocyte and embryo development
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00543949" target="_blank" >RIV/67985904:_____/21:00543949 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S2468054021000202?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2468054021000202?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ncrna.2021.06.001" target="_blank" >10.1016/j.ncrna.2021.06.001</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Oocyte specific lncRNA variant Rose influences oocyte and embryo development
Popis výsledku v původním jazyce
Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, Rose (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development.
Název v anglickém jazyce
Oocyte specific lncRNA variant Rose influences oocyte and embryo development
Popis výsledku anglicky
Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, Rose (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
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OECD FORD obor
10605 - Developmental biology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA18-19395S" target="_blank" >GA18-19395S: Role nekódujících RNA v savčím oocytu a embryu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Non-coding RNA research
ISSN
2468-2160
e-ISSN
2468-2160
Svazek periodika
6
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CN - Čínská lidová republika
Počet stran výsledku
7
Strana od-do
107-113
Kód UT WoS článku
000670265100007
EID výsledku v databázi Scopus
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