The Expression of Selected Wnt Pathway Members (FZD6, AXIN2 and beta-Catenin) in Canine Oral Squamous Cell Carcinoma and Acanthomatous Ameloblastoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00544013" target="_blank" >RIV/67985904:_____/21:00544013 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/21:43879110

Výsledek na webu

<a href="https://www.mdpi.com/2076-2615/11/6/1615" target="_blank" >https://www.mdpi.com/2076-2615/11/6/1615</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani11061615" target="_blank" >10.3390/ani11061615</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Expression of Selected Wnt Pathway Members (FZD6, AXIN2 and beta-Catenin) in Canine Oral Squamous Cell Carcinoma and Acanthomatous Ameloblastoma

Popis výsledku v původním jazyce

Simple Summary The expression patterns of selected Wnt pathway molecules were analyzed in two of the most common canine oral neoplasia-canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). We found an overlap of areas with high expression of FZD6 and SOX2 in COSCC, while cytokeratin expression was low in these areas, indicating the low differentiation status of these cells. In CAA, FZD6-positive areas expressed cytokeratin and exhibited features of squamous metaplasia. Moreover, the expression of beta-catenin and AXIN2 was higher in both CAA and COSCC than in the healthy canine oral epithelium. This work uncovered the distinct expression patterns of Wnt molecules in both lesions, indicating the involvement of this pathway in the pathology of canine oral cancers, which presents opportunities for their usage for the prediction of cell behavior or in the development of new therapeutic approaches. The Wnt signaling pathway is well known to be involved in many types of human cancer, however, in veterinary medicine, the investigation of individual Wnt members' expression, and their role in or association with oral tumor pathogenesis, is still underevaluated. We aim to determine the expression pattern of Frizzled-6 (FZD6) as one of the Wnt receptors in two of the most common canine oral neoplastic lesions-canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). While COSCC is a malignant tumor with aggressive biological behavior and a tendency to metastasize, CAA is a benign tumor with high local invasiveness. In CAA, the expression of FZD6 was mostly located in the center of the epithelial tumorous tissue, and cells exhibiting features of squamous metaplasia were strongly positive. In well-differentiated COSCC, FZD6 was expressed in the tumorous epithelium as well as the tumorous stroma. There was a negative correlation between cytokeratin expression and FZD6 expression in COSCC, where the central parts of the epithelial tumorous tissue were often FZD6-negative. The non-differentiated COSCC with low expression of cytokeratin exhibited a diffuse FZD6 signal. The invasive front with areas of tumor budding exhibited high FZD6 expression with a loss of cytokeratin expression. Moreover, the expression of beta-catenin and AXIN2 was increased in comparison to gingiva. In conclusion, our study revealed significant differences in the expression patterns and the levels of FZD6 between COSCC and CAA, indicating the differential engagement of the Wnt pathway in these tumors.

Název v anglickém jazyce

The Expression of Selected Wnt Pathway Members (FZD6, AXIN2 and beta-Catenin) in Canine Oral Squamous Cell Carcinoma and Acanthomatous Ameloblastoma

Popis výsledku anglicky

Simple Summary The expression patterns of selected Wnt pathway molecules were analyzed in two of the most common canine oral neoplasia-canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). We found an overlap of areas with high expression of FZD6 and SOX2 in COSCC, while cytokeratin expression was low in these areas, indicating the low differentiation status of these cells. In CAA, FZD6-positive areas expressed cytokeratin and exhibited features of squamous metaplasia. Moreover, the expression of beta-catenin and AXIN2 was higher in both CAA and COSCC than in the healthy canine oral epithelium. This work uncovered the distinct expression patterns of Wnt molecules in both lesions, indicating the involvement of this pathway in the pathology of canine oral cancers, which presents opportunities for their usage for the prediction of cell behavior or in the development of new therapeutic approaches. The Wnt signaling pathway is well known to be involved in many types of human cancer, however, in veterinary medicine, the investigation of individual Wnt members' expression, and their role in or association with oral tumor pathogenesis, is still underevaluated. We aim to determine the expression pattern of Frizzled-6 (FZD6) as one of the Wnt receptors in two of the most common canine oral neoplastic lesions-canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). While COSCC is a malignant tumor with aggressive biological behavior and a tendency to metastasize, CAA is a benign tumor with high local invasiveness. In CAA, the expression of FZD6 was mostly located in the center of the epithelial tumorous tissue, and cells exhibiting features of squamous metaplasia were strongly positive. In well-differentiated COSCC, FZD6 was expressed in the tumorous epithelium as well as the tumorous stroma. There was a negative correlation between cytokeratin expression and FZD6 expression in COSCC, where the central parts of the epithelial tumorous tissue were often FZD6-negative. The non-differentiated COSCC with low expression of cytokeratin exhibited a diffuse FZD6 signal. The invasive front with areas of tumor budding exhibited high FZD6 expression with a loss of cytokeratin expression. Moreover, the expression of beta-catenin and AXIN2 was increased in comparison to gingiva. In conclusion, our study revealed significant differences in the expression patterns and the levels of FZD6 between COSCC and CAA, indicating the differential engagement of the Wnt pathway in these tumors.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/NV19-08-00383" target="_blank" >NV19-08-00383: Prognostické a prediktivní markery invaze spinocelulárních karcinomů dutiny ústní a orofaryngu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animals

ISSN

2076-2615

e-ISSN

2076-2615

Svazek periodika

11

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

1615

Kód UT WoS článku

000667025200001

EID výsledku v databázi Scopus

2-s2.0-85106679623